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Knowledge Base

[Readers Insight] Why Peak Fronting Occurs? Causes and Analysis

April 14, 2026

Peak fronting is a common chromatographic anomaly characterized by a gradual leading edge and a steep trailing edge, often occurring during method development. This phenomenon stems from multiple causes, thus resolving it can be much more difficult. In this article, we discuss three primary sources of peak fronting and provide technical solutions to mitigate these effects and optimize chromatographic performance.

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[Readers Insight] When to Use Acetone as a Mobile Phase?

April 12, 2026

Acetone is a versatile solvent in chromatography, yet its application as an HPLC mobile phase remains limited to specific cases like Sudan dye analysis. This article explores the reasons behind the rare use of acetone and summarizes the key considerations when using acetone as a mobile phase.

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[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories

March 30, 2026

We all know that Mass Spectrometry is preferable for trace analysis due to its superior sensitivity. But why do PDA detectors still maintain a high utilization rate? In this article, we explore the practical advantages PDA holds compared to MS.

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The Selectivity Among Detectors: Why Mass Spectrometry Surpasses Spectroscopy?

March 26, 2026

Selectivity is a definitive factor in analytical chemistry. In this article, we discuss the differences between optical spectra and mass spectra, and explain why mass spectrometry surpasses spectroscopy in selectivity.

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[Readers Insight] Is "Not Detected" Truly "Not Detected"? (Not An Article about LOD)

March 19, 2026

When you encounter "Not Detected" in analytical chemistry, does it truly mean the substance is absent? In practice, unscrupulous manufacturers may try to evade detection of prohibited additives by modifying molecular structures to something out of the detection scope. In this article, the author introduces a method using TOF-IDA/MS to effectively expose such modification without the need of reference standards.

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[Readers Insight] How to Analyze Fragment Ions in Mass Spectrometry?

January 19, 2026

In this article, we investigate the fundamental mechanisms of ion fragmentation within mass spectrometry workflows and explore why systematically mapping fragment-ion structures is essential for deeper mass-spectrometric interpretation. It explores the relationship between electronic displacement and dissociation pathways, emphasizing structural reconstruction over simple pattern recognition.

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[Readers Insight] The Interplay between Column Properties and Peak Integration

January 14, 2026

In HPLC, the relationship between physical separation hardware and mathematical data processing is often overlooked. This article explores the inconspicuous but critical connection between column properties and the fidelity of peak area calculation, examining why theoretical constancy in integration often fails to align with empirical laboratory observations.

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[Readers Insight] Gradient vs. Isocratic Elution: Which to Choose?

January 6, 2026

In this article, the author evaluates isocratic and gradient elution strategies in HPLC method development, providing expert guidance on selecting elution modes for pharmaceutical assays, impurity profiling, and complex separations.

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[Readers Insight] Why is Formic Acid Added to the Mobile Phase in Mass Spectrometry?

December 11, 2025

Formic acid is commonly seen added to the mobile phase in mass spectrometry methods, but have you wondered what specific role it plays, and can other acids substitute it? This article explains the role of formic acid in MS from the perspective of chemical reaction equilibrium thoroughly.

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[Readers Insight] Heterocycles Structural Analysis in HPLC Method Development

December 1, 2025

In this article, the author analyzes the structures of heterocycles (mostly aromatic heterocycles) and how the structures affect heterocycles' acid-base property.

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[Readers Insight] Choose Methanol or Acetonitrile? The Organic Mobile Phase in HPLC

November 17, 2025

In HPLC analysis, methanol and acetonitrile are the most common organic mobile phases. But have you ever thought about what exactly differentiates them? When should methanol be used and when acetonitrile? In this article, the author shares their thoughts on this matter.

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[Readers Insight] Why Do Substances with Similar Polarity Dissolve in Each Other?

November 12, 2025

In this article and attached video, the author discusses why substances with similar polarity dissolve in each other through dipole-dipole interactions, the energy change during dissolution, and the effect from entropy and Gibbs free energy.

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