Knowledge Base

In modern analytical chemistry, UHPLC offers significant benefits, including increased throughput, superior resolution, and reduced solvent consumption. However, transferring methods from HPLC to UHPLC is more than simply swapping columns. In this article, we explore the essentials in transferring a method, from mathematical calculation to method validation.

HPLC has been one of the most widely used analytical chromatographic techniques, yet it is not fast enough to meet every laboratory analysis. In this article, we explore UHPLC, the faster version of liquid chromatography, compare the differences between UHPLC and HPLC, and discuss the limitations and application fields of UHPLC.

The existence of hydrogen bonding in acetonitrile-water solutions is a fundamental question. In this article, we will discuss the question in details, including the physicochemical properties of acetonitrile and water, the interaction that takes place when acetonitrile is dissolved in water, and the abnormalities that may occur when using acetonitrile-water solutions as mobile phase or for sample extraction.

Poor resolution is challenging, but elution order reversal is even perplexing. In this article, the author discusses three primary factors that cause peaks to elute inversed when mobile phase changes: pH, organic modifier, and buffer salt.

Reproducibility issues are an unavoidable topic in HPLC. In this article, we break down easily overlooked, application-level factors that cause reproducibility failures into four aspects: reagents, samples, instruments, and columns, and provide a practical troubleshooting framework to help chromatographers isolate issues and achieve robust, consistent separation results in daily laboratory practice.

Unexplained peak fronting and split peaks often compromise HPLC data quality. Among all possible culprits, solvent effect is the one often overlooked. This article explores the mechanisms of this hidden killer and provides actionable strategies to restore peak symmetry.

In this article, we discuss the catastrophic results one would get if they replace formic acid in the mobile phase by acetic acid in an HPLC experiment.

In this article, we explore a novel analytical approach utilizing the ionic liquid [EimHSO4] for copper ion extraction. By examining its unique phase separation and coordination mechanisms, it highlights how this innovative methodology could pave the way for future LC-MS sample preparation and quantification.

In laboratory analysis, it is common to encounter scenarios where a column loses efficacy after being used in a different project, or where dozens of projects must be conducted by a small amount of columns. In this article, we explore the balance of dedicated and shared HPLC column use to ensure reliable analytical results while maximizing available resources.

Peak fronting is a common chromatographic anomaly characterized by a gradual leading edge and a steep trailing edge, often occurring during method development. This phenomenon stems from multiple causes, thus resolving it can be much more difficult. In this article, we discuss three primary sources of peak fronting and provide technical solutions to mitigate these effects and optimize chromatographic performance.

Acetone is a versatile solvent in chromatography, yet its application as an HPLC mobile phase remains limited to specific cases like Sudan dye analysis. This article explores the reasons behind the rare use of acetone and summarizes the key considerations when using acetone as a mobile phase.

We all know that Mass Spectrometry is preferable for trace analysis due to its superior sensitivity. But why do PDA detectors still maintain a high utilization rate? In this article, we explore the practical advantages PDA holds compared to MS.