Knowledge Base

[Readers Insight] Matrix Effects in Mass Spectrometry: Why They Matter
by Shu Cheng
September 22, 2025

In this article, we explore further how matrix effects affects recovery results in mass spectrometry analyses by hindering preparation loss, and discuss when it should be corrected by using calibration curves prepared by blank matrices.

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[Reader Insights] Acidic or Basic? Read Analyte Structure First When Developing a Method
by Shu Cheng
September 11, 2025

In this article, we discuss how to read molecular structure to assess an analyte’s acidic or basic character and use that insight to design starting chromatographic methods. You will find practical, rule-based criteria, clear decision points for method setup, and a focused real-world example that illustrates structure-driven choices.

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[Readers Insight] The Starting Point in Method Development
by Shu Cheng
August 24, 2025

Method development often begins with uncertainty—especially when faced with unknown compounds and no literature guidance. This article examines a pharmacopoeial method as a case study, highlighting its chromatographic settings, analyzing potential limitations, and considering improvements. Through this, readers gain insight into the logical framework behind choosing starting conditions.

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[Readers Insight] Are You Choosing the Right Quantifier Ion for Mass Spectrometry?
by Shu Cheng
August 20, 2025

In this article, the author discusses the principles behind selecting quantifier ions in triple quadrupole mass spectrometry, with a case study to illustrate how ion choice impacts sensitivity and accuracy.

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[Readers Insight] Why Do M-Shaped Peaks Occur?
by Shu Cheng
August 14, 2025

M-shaped peaks can puzzle even experienced chromatographers, appearing unexpectedly and resisting simple fixes. This article draws on expert insights to explore their possible origins and practical approaches, guiding analysts toward more reliable and consistent chromatographic results.

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Agarose: A Versatile Packing Material for Protein Purification
by Shu Cheng
August 5, 2025

Agarose-based chromatographic media offer a compelling blend of biocompatibility, versatility, and affordability. By mastering the principles of agarose microsphere preparation and crosslinking, chromatographers can tailor supports to a vast array of protein purification challenges, ensuring high yield, purity, and process robustness.

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Mastering Gas Chromatography in 7 Comprehensive Steps!
by Shu Cheng
July 28, 2025

This article guides analysts in GC analyses through efficient sample preparation, instrument configuration, initial condition setup, separation optimization, qualitative identification using retention indices or GC–MS, quantitative analysis via internal standards or standard addition, rigorous method validation, ensuring accurate, precise, reproducible results across diverse unknown sample analyses consistently.

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Choosing Gas Chromatography Columns: Key Parameters
by Shu Cheng
July 24, 2025

In gas chromatography, column length, internal diameter, and film thickness critically determine efficiency, retention times, pressure requirements, and sample capacity. This article reviews how each parameter impacts chromatographic performance and provides practical selection guidelines—balancing resolution, analysis time, and cost—to help analysts choose optimal capillary columns for diverse applications.

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Uncovering Overlooked Factors Behind Abnormal Baselines
by Shu Cheng
July 23, 2025

A stable, flat baseline is essential for accurate HPLC analyses, yet analysts may grapple with noise and drift. This article explores key baseline disturbances—sawtooth patterns, pulsations, chaotic fluctuations, and drift—pinpoints their root causes, and offers precise remedies. A real-world case study demonstrates systematic troubleshooting to restore baseline integrity.

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