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Knowledge Base

[Readers Insight] How to Analyze Fragment Ions in Mass Spectrometry?

January 19, 2026

In this article, we investigate the fundamental mechanisms of ion fragmentation within mass spectrometry workflows and explore why systematically mapping fragment-ion structures is essential for deeper mass-spectrometric interpretation. It explores the relationship between electronic displacement and dissociation pathways, emphasizing structural reconstruction over simple pattern recognition.

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[Readers Insight] The Interplay between Column Properties and Peak Integration

January 14, 2026

In HPLC, the relationship between physical separation hardware and mathematical data processing is often overlooked. This article explores the inconspicuous but critical connection between column properties and the fidelity of peak area calculation, examining why theoretical constancy in integration often fails to align with empirical laboratory observations.

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[Readers Insight] Gradient vs. Isocratic Elution: Which to Choose?

January 6, 2026

In this article, the author evaluates isocratic and gradient elution strategies in HPLC method development, providing expert guidance on selecting elution modes for pharmaceutical assays, impurity profiling, and complex separations.

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[Readers Insight] Why is Formic Acid Added to the Mobile Phase in Mass Spectrometry?

December 11, 2025

Formic acid is commonly seen added to the mobile phase in mass spectrometry methods, but have you wondered what specific role it plays, and can other acids substitute it? This article explains the role of formic acid in MS from the perspective of chemical reaction equilibrium thoroughly.

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[Readers Insight] Heterocycles Structural Analysis in HPLC Method Development

December 1, 2025

In this article, the author analyzes the structures of heterocycles (mostly aromatic heterocycles) and how the structures affect heterocycles' acid-base property.

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[Readers Insight] Choose Methanol or Acetonitrile? The Organic Mobile Phase in HPLC

November 17, 2025

In HPLC analysis, methanol and acetonitrile are the most common organic mobile phases. But have you ever thought about what exactly differentiates them? When should methanol be used and when acetonitrile? In this article, the author shares their thoughts on this matter.

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[Readers Insight] Why Do Substances with Similar Polarity Dissolve in Each Other?

November 12, 2025

In this article and attached video, the author discusses why substances with similar polarity dissolve in each other through dipole-dipole interactions, the energy change during dissolution, and the effect from entropy and Gibbs free energy.

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[Readers Insight] Why Do Ghost Peaks Appear?

October 31, 2025

In this article, we examine the common origins of ghost peaks in HPLC. From mobile phase contamination and instrument residues, to column impurities and sample-related degradation, the article explains how they contribute to the appearance of ghost peaks.

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[Readers Insight] Is Retention Time Determined Solely by the Polarity of a Compound?

October 27, 2025

Have you ever thought about this question: In reversed-phase chromatography, does an earlier elution always mean the compound has higher polarity? Or rather — is retention time determined solely by the polarity of a compound? In this article, we explore what polarity is, why it acts as the primary factor affecting retention time, and what other factors are unneglectable.

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[Readers Insight] Mobile Phase Selection in Method Development: How to Optimize

October 21, 2025

In method development, the selection of mobile phases is an important part to success. This article discusses thoroughly different mobile phases and their use in HPLC and LC-MS, and provides recommendations for initial mobile phase choices.

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Common Problems in Solid-Phase Extraction: A Practical Troubleshooting Guide

October 17, 2025

Solid-Phase Extrtaction is a fast, easy and high-performance sample preparation technique widely used, but routine SPE runs can encounter recurring problems. This article summarizes typical causes and practical fixes for each SPE problem.

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[Readers Insight] Retention Time Drifts: Why Do They Occur?

October 17, 2025

Learn the causes of retention time drift in HPLC analyses, explained thoroughly through mobile phase, column, instrument, and sample solvent.

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