Knowledge Base

Advancing Metabolic Therapeutics: An Overview of Retatrutide and the Analytical Rigor of Peptide Quantification

February 9, 2026

Retatrutide is a new synthetic peptide that facilitates weight loss through a dual-action mechanism. In this article, we utilize an Ultisil LP-C18 column for the determination of Retatrutide that achieves baseline separation with high resolution records.

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[Readers Insight] How to Analyze Fragment Ions in Mass Spectrometry?

January 19, 2026

In this article, we investigate the fundamental mechanisms of ion fragmentation within mass spectrometry workflows and explore why systematically mapping fragment-ion structures is essential for deeper mass-spectrometric interpretation. It explores the relationship between electronic displacement and dissociation pathways, emphasizing structural reconstruction over simple pattern recognition.

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[Readers Insight] Asking the Questions Right: A Guide to Troubleshooting Consultations in HPLC Method Development

January 15, 2026

Mastering HPLC method development requires more than technical skill; it demands effective communication. This guide explores how junior scientists can overcome "question anxiety" by utilizing precise data packages and structured inquiries. Learn to transform troubleshooting consultations into professional growth opportunities, ensuring faster resolutions and building lasting laboratory expertise.

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[Readers Insight] The Interplay between Column Properties and Peak Integration

January 14, 2026

In HPLC, the relationship between physical separation hardware and mathematical data processing is often overlooked. This article explores the inconspicuous but critical connection between column properties and the fidelity of peak area calculation, examining why theoretical constancy in integration often fails to align with empirical laboratory observations.

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[Readers Insight] Gradient vs. Isocratic Elution: Which to Choose?

January 6, 2026

In this article, the author evaluates isocratic and gradient elution strategies in HPLC method development, providing expert guidance on selecting elution modes for pharmaceutical assays, impurity profiling, and complex separations.

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LP Columns: More Than Just Tolerating Strong Acids

December 31, 2025

Ultisil LP columns from Welch Materials are designed for low-pH chromatographic conditions, yet their advantages are more than simply resistance to strong acids. Read this article to learn how these columns outperform in a unique way.

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High-Resolution SEC-HPLC Aggregation Analysis of a Pembrolizumab Biosimilar Using Welch Xtimate™ SEC Columns

December 29, 2025

This application note presents a robust SEC-HPLC method for high-resolution aggregation analysis of a Pembrolizumab biosimilar using Welch Xtimate™ SEC-300 columns. The method demonstrates excellent separation of monomer, HMW aggregates, and LMW fragments, supporting routine QC and biosimilar comparability studies.

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[Readers Insight] Hydrophilic Interaction Chromatography (HILIC): A Nemesis to Polar Compounds

December 23, 2025

Polar compounds that refuse to stay on C18 columns can be frustrating. HILIC chromatography provides a versatile solution through partitioning and interaction mechanisms. In this post, we break down HILIC principles, column types, mobile phase strategies, and key method development tips to help you achieve reliable retention and separation.

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The Flow Cell in HPLC: A Small Component with Big Consequences

December 19, 2025

In LC analysis, column, mobile phase, and gradient are what we pay most attention to. However, sometimes the difference in flow cells can lead to significantly different results. This article explains the details behind it.

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[Readers Insight] Triethylamine as a Mobile Phase Additive: What Does It Do?

December 15, 2025

In this article, the author explains the uses of triethylamine as a mobile phase additive in HPLC, with real-world examples from pharmacopoeias and literatural applications.

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[Readers Insight] Why is Formic Acid Added to the Mobile Phase in Mass Spectrometry?

December 11, 2025

Formic acid is commonly seen added to the mobile phase in mass spectrometry methods, but have you wondered what specific role it plays, and can other acids substitute it? This article explains the role of formic acid in MS from the perspective of chemical reaction equilibrium thoroughly.

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Stop Flushing Arbitrarily! These Procedures Effectively Regenerates Your HPLC Columns

December 4, 2025

Inadequate column flushing causes HPLC columns to show abnormalities quickly even after only a few runs. In this article, we put together how to perform routine and regeneration flushing of Welch HPLC columns and store them correctly to extend their lives.

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