Knowledge Base

In modern analytical chemistry, UHPLC offers significant benefits, including increased throughput, superior resolution, and reduced solvent consumption. However, transferring methods from HPLC to UHPLC is more than simply swapping columns. In this article, we explore the essentials in transferring a method, from mathematical calculation to method validation.

HPLC has been one of the most widely used analytical chromatographic techniques, yet it is not fast enough to meet every laboratory analysis. In this article, we explore UHPLC, the faster version of liquid chromatography, compare the differences between UHPLC and HPLC, and discuss the limitations and application fields of UHPLC.

Chromatographic analysis for peptide drugs are important for ensuring product quality and safety. In this article, we introduce our solutions for peptide drug analysis, including the chiral analysis of protected amino acids, the determination of peptide related substances, the analysis of peptide aggregates by SEC, and the evaluation of amino acid ratios using the Welch Ultisil Amino Acid Method Kit.

The existence of hydrogen bonding in acetonitrile-water solutions is a fundamental question. In this article, we will discuss the question in details, including the physicochemical properties of acetonitrile and water, the interaction that takes place when acetonitrile is dissolved in water, and the abnormalities that may occur when using acetonitrile-water solutions as mobile phase or for sample extraction.

Chocolate is one of the most consumed sweet on Earth, and its quality and safety is a major consideration. However, chocolate's component greatly interfere with chromatographic analysis. In this article, we present an HPLC method with trichloromethane pretreatment to analyze Disodium EDTA in chocolate.

In this article, we present solutions for the chromatographic analysis of biological samples from Welch Materials, covering SEC, RPLC, HIC, and ion exchange chromatography, with multiple applications of peptides, nucleic acids, antibodies, and proteins.

Poor resolution is challenging, but elution order reversal is even perplexing. In this article, the author discusses three primary factors that cause peaks to elute inversed when mobile phase changes: pH, organic modifier, and buffer salt.

Reproducibility issues are an unavoidable topic in HPLC. In this article, we break down easily overlooked, application-level factors that cause reproducibility failures into four aspects: reagents, samples, instruments, and columns, and provide a practical troubleshooting framework to help chromatographers isolate issues and achieve robust, consistent separation results in daily laboratory practice.

Unexplained peak fronting and split peaks often compromise HPLC data quality. Among all possible culprits, solvent effect is the one often overlooked. This article explores the mechanisms of this hidden killer and provides actionable strategies to restore peak symmetry.

Dynamic Axial Compression (DAC) columns are a definitive tool in preparative-scale liquid chromatographic separation and purification. In this article, we thoroughly explore the key advantages of WelPacker DAC and discover its packing and maintenance details.

In this article, we discuss the catastrophic results one would get if they replace formic acid in the mobile phase by acetic acid in an HPLC experiment.

In this article, we explore a novel analytical approach utilizing the ionic liquid [EimHSO4] for copper ion extraction. By examining its unique phase separation and coordination mechanisms, it highlights how this innovative methodology could pave the way for future LC-MS sample preparation and quantification.