Knowledge Base
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Maximizing Longevity: Five Practical Ways to Extend HPLC Column Life
An HPLC column is a high-cost core consumable in laboratories, and improper handling and operational errors contribute to column degration. In this article, we introduce five foundational rules that safeguard your HPLC columns and explain the reason behind it. -
Advanced Chromatographic Resolution of Chiral Compounds, Part V: APIs
More than half of APIs contain chiral centers, which may hinder drug activity if not separated. In this article, we use Blossmate chiral columns to separate five distinct APIs with their enantiomers, showcasing the columns' capability in such applications. -
A Simple and Efficient Solution for HPLC Sensitivity: The WelView Photo Chemical Reactor
In chromatographic analysis, achieving high sensitivity and selectivity for trace-level analytes within complex matrices is a foundational challenge. In this article, we introduce WelView Photo Chemical Reactor and explore how it enhances HPLC sensitivity with post-column derivatization through two examples. -
A Comparison of Welch Ultisil Columns on the Isomeric Separation of Anthraquinones
Isomer separation is one of the most challenging tasks in chromatography. In this article, we evaluate the chromatographic performance of three Welch Materials’ Ultisil stationary phases—PFP, Alk-C18, and XS-C18—on the separation of isomeric derivatives and analogs of antharaquinone. -
Hydrophobic Interaction Chromatography: Your Best Teammate in the Analysis of ADCs
In the analysis of antibody-drug conjugates, the determination of drug-to-antibody ratio is necessary. In this article, we explain why hydrophobic interaction chromatography excels in DAR determination and how to choose the column & detector and optimize the mobile phase. -
Advanced Chromatographic Resolution of Chiral Compounds, Part IV: Herbicides
Herbicides are an essential tool in weed management, and chirality greatly affects their efficacy. In this article, we explore the chiral separation of two herbicides: 2-(2-chlorophenoxy)propanoic acid and dimethenamid, using Welch Blossmate chiral columns. -
Dedicate or Share? Maximize Your HPLC Column Use
In laboratory analysis, it is common to encounter scenarios where a column loses efficacy after being used in a different project, or where dozens of projects must be conducted by a small amount of columns. In this article, we explore the balance of dedicated and shared HPLC column use to ensure reliable analytical results while maximizing available resources. -
Advanced Chromatographic Resolution of Chiral Compounds, Part III: Alcohols and Diols
Alcohols and diols are among the most common functionalized intermediates in organic synthesis and pharmaceutical development, and chirality can influence their efficacy greatly. In this article, we introduce applications on the chiral separation of several typical alcohols and diol using Blossmate chiral columns. -
Mitigating Non-Specific Adsorption in Size Exclusion Chromatography: The Welch Xtimate Bio SEC Solution
Biopharmaceutical integrity relies on precise molecular size characterization. In this article, we explain how Welch Xtimate Bio SEC columns mitigate non-specific adsorption in SEC compared with competitors, and provide guides on optimizing mobile phase in different SEC assays. -
Advanced Chromatographic Resolution of Chiral Compounds, Part II: Amino Acids
Amino acids and their derivatives serve as fundamental building blocks in the synthesis of bioactive peptides and chiral drugs, and their chirality greatly affect the quality of the final product. In this article, we introduce the enantiomer separation of four different amino acid derivatives with Welch Blossmate chiral columns. -
Advanced Chromatographic Resolution of Chiral Compounds, Part I: NSAIDs
Achieving enantiomeric purity is vital for the clinical safety and efficacy. Among the most widely prescribed analgesics, NSAIDs presents a persistent analytical challenge due to the subtle structural variations between their R- and S-enantiomers. In this article, we present the chiral separation of three significant "profen" NSAIDs (flurbiprofen, tiaprofenic acid, and carprofen) with Welch's new Blossmate chiral columns. -
![[Readers Insight] Why Peak Fronting Occurs? Causes and Analysis](//www.welch-us.com/cdn/shop/articles/fronting-414-1080_535x.jpg?v=1776216534)
![[Readers Insight] Why Peak Fronting Occurs? Causes and Analysis](//www.welch-us.com/cdn/shop/articles/fronting-414-1080_345x345@2x.jpg?v=1776216534)
[Readers Insight] Why Peak Fronting Occurs? Causes and Analysis
Peak fronting is a common chromatographic anomaly characterized by a gradual leading edge and a steep trailing edge, often occurring during method development. This phenomenon stems from multiple causes, thus resolving it can be much more difficult. In this article, we discuss three primary sources of peak fronting and provide technical solutions to mitigate these effects and optimize chromatographic performance.
![[Readers Insight] Why Peak Fronting Occurs? Causes and Analysis](http://www.welch-us.com/cdn/shop/articles/fronting-414-1080_535x.jpg?v=1776216534)
![[Readers Insight] Why Peak Fronting Occurs? Causes and Analysis](http://www.welch-us.com/cdn/shop/articles/fronting-414-1080_345x345@2x.jpg?v=1776216534)