Knowledge Base

Chirality, the handedness in chemistry, can mean a significant difference between life-saver and toxicity. In this article, we not only explore how chiral HPLC separates enantiomers with its unique three-point interaction mechanism, but also briefly introduce five brand new chiral stationary phases in Blossmate Chiral series, greatly expanding Welch Materials' chiral chromatography solutions.

In analytical chemistry, spiked recovery rate is a fundamental parameter used to evaluate the accuracy of an analytical method. In this article, we explore the three categories of spiked recovery experiments, the calculation of spiked recovery rate and required spiking amount, and the practical experimental guidelines for a valid experiment.

The sugar column used in carbohydrate and sugar analysis has different operational precautions than other commonly used chromatographic columns. Many users, even after reading the product manual, still have difficulties on how to properly activate, use, and store them. In this article, we break down the details of using sugar columns

In introductory liquid chromatography training, almost every beginner has heard the following statement: “In reversed-phase chromatography, more polar compounds elute first, while less polar compounds elute later.” But chromatography is not that simple. In this article, we discuss factors beyond polarity that determines elution order.

Cereulide is an emetic toxin found in grains and dairy products that requires prevention and control. In this article, we present a method that quantifies cereulide in infant formula and rice cereal, using solid-phase extraction, UHPLC, and mass spectrometry.

In HPLC, temperature is an often overlooked factor. Without temperature control, methods may suffer from many abnormalities. In this article, we discuss how temperature influences chromatographic behavior and how column ovens play a role in performance.

In modern analytical chemistry, UHPLC offers significant benefits, including increased throughput, superior resolution, and reduced solvent consumption. However, transferring methods from HPLC to UHPLC is more than simply swapping columns. In this article, we explore the essentials in transferring a method, from mathematical calculation to method validation.

HPLC has been one of the most widely used analytical chromatographic techniques, yet it is not fast enough to meet every laboratory analysis. In this article, we explore UHPLC, the faster version of liquid chromatography, compare the differences between UHPLC and HPLC, and discuss the limitations and application fields of UHPLC.

Chromatographic analysis for peptide drugs are important for ensuring product quality and safety. In this article, we introduce our solutions for peptide drug analysis, including the chiral analysis of protected amino acids, the determination of peptide related substances, the analysis of peptide aggregates by SEC, and the evaluation of amino acid ratios using the Welch Ultisil Amino Acid Method Kit.

The existence of hydrogen bonding in acetonitrile-water solutions is a fundamental question. In this article, we will discuss the question in details, including the physicochemical properties of acetonitrile and water, the interaction that takes place when acetonitrile is dissolved in water, and the abnormalities that may occur when using acetonitrile-water solutions as mobile phase or for sample extraction.

Chocolate is one of the most consumed sweet on Earth, and its quality and safety is a major consideration. However, chocolate's component greatly interfere with chromatographic analysis. In this article, we present an HPLC method with trichloromethane pretreatment to analyze Disodium EDTA in chocolate.

In this article, we present solutions for the chromatographic analysis of biological samples from Welch Materials, covering SEC, RPLC, HIC, and ion exchange chromatography, with multiple applications of peptides, nucleic acids, antibodies, and proteins.