Knowledge Base
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![[Readers Insight] Why Mobile Phase Alterations Can Reverse Elution Order](//www.welch-us.com/cdn/shop/articles/elution-order-630-1080_fa63e819-5a63-4bdd-8451-041cbce9a310_535x.jpg?v=1782971026)
![[Readers Insight] Why Mobile Phase Alterations Can Reverse Elution Order](//www.welch-us.com/cdn/shop/articles/elution-order-630-1080_fa63e819-5a63-4bdd-8451-041cbce9a310_345x345@2x.jpg?v=1782971026)
[Readers Insight] Why Mobile Phase Alterations Can Reverse Elution Order
Poor resolution is challenging, but elution order reversal is even perplexing. In this article, the author discusses three primary factors that cause peaks to elute inversed when mobile phase changes: pH, organic modifier, and buffer salt. -
When LC Methods Failed to Reproduce: What Could Be the Cause?
Reproducibility issues are an unavoidable topic in HPLC. In this article, we break down easily overlooked, application-level factors that cause reproducibility failures into four aspects: reagents, samples, instruments, and columns, and provide a practical troubleshooting framework to help chromatographers isolate issues and achieve robust, consistent separation results in daily laboratory practice. -
Solvent Effect, The Hidden Killer of Chromatographic Peak Shapes
Unexplained peak fronting and split peaks often compromise HPLC data quality. Among all possible culprits, solvent effect is the one often overlooked. This article explores the mechanisms of this hidden killer and provides actionable strategies to restore peak symmetry. -
WelPacker DAC: Your Best Teammate in Purification
Dynamic Axial Compression (DAC) columns are a definitive tool in preparative-scale liquid chromatographic separation and purification. In this article, we thoroughly explore the key advantages of WelPacker DAC and discover its packing and maintenance details. -
![[Readers Insight] What Happens If Formic Acid Was Replaced by Acetic Acid in HPLC Analysis?](//www.welch-us.com/cdn/shop/articles/acetic-acid-1080_535x.jpg?v=1781761748)
![[Readers Insight] What Happens If Formic Acid Was Replaced by Acetic Acid in HPLC Analysis?](//www.welch-us.com/cdn/shop/articles/acetic-acid-1080_345x345@2x.jpg?v=1781761748)
[Readers Insight] What Happens If Formic Acid Was Replaced by Acetic Acid in HPLC Analysis?
In this article, we discuss the catastrophic results one would get if they replace formic acid in the mobile phase by acetic acid in an HPLC experiment. -
![[Readers Insight] A Novel Analytical Approach: New Perspectives on Ionic Liquid Extraction](//www.welch-us.com/cdn/shop/articles/1080-615_00fd03ec-e50b-4a24-ac8d-bb3f85408d1a_535x.jpg?v=1781595867)
![[Readers Insight] A Novel Analytical Approach: New Perspectives on Ionic Liquid Extraction](//www.welch-us.com/cdn/shop/articles/1080-615_00fd03ec-e50b-4a24-ac8d-bb3f85408d1a_345x345@2x.jpg?v=1781595867)
[Readers Insight] A Novel Analytical Approach: New Perspectives on Ionic Liquid Extraction
In this article, we explore a novel analytical approach utilizing the ionic liquid [EimHSO4] for copper ion extraction. By examining its unique phase separation and coordination mechanisms, it highlights how this innovative methodology could pave the way for future LC-MS sample preparation and quantification. -
Advanced Chromatographic Resolution of Chiral Compounds, Part VI: Intermediates
While synthetic intermediates are not themselves in the final API, their stereochemical purity affects product quality greatly. In this article, we present application notes of five typical synthetic intermediates, witnessing how Blossmate chiral columns successfully separate them from their enantiomers. -
Maximizing Longevity: Five Practical Ways to Extend HPLC Column Life
An HPLC column is a high-cost core consumable in laboratories, and improper handling and operational errors contribute to column degration. In this article, we introduce five foundational rules that safeguard your HPLC columns and explain the reason behind it. -
Advanced Chromatographic Resolution of Chiral Compounds, Part V: APIs
More than half of APIs contain chiral centers, which may hinder drug activity if not separated. In this article, we use Blossmate chiral columns to separate five distinct APIs with their enantiomers, showcasing the columns' capability in such applications. -
A Simple and Efficient Solution for HPLC Sensitivity: The WelView Photo Chemical Reactor
In chromatographic analysis, achieving high sensitivity and selectivity for trace-level analytes within complex matrices is a foundational challenge. In this article, we introduce WelView Photo Chemical Reactor and explore how it enhances HPLC sensitivity with post-column derivatization through two examples. -
A Comparison of Welch Ultisil Columns on the Isomeric Separation of Anthraquinones
Isomer separation is one of the most challenging tasks in chromatography. In this article, we evaluate the chromatographic performance of three Welch Materials’ Ultisil stationary phases—PFP, Alk-C18, and XS-C18—on the separation of isomeric derivatives and analogs of antharaquinone. -
Hydrophobic Interaction Chromatography: Your Best Teammate in the Analysis of ADCs
In the analysis of antibody-drug conjugates, the determination of drug-to-antibody ratio is necessary. In this article, we explain why hydrophobic interaction chromatography excels in DAR determination and how to choose the column & detector and optimize the mobile phase.
![[Readers Insight] Why Mobile Phase Alterations Can Reverse Elution Order](http://www.welch-us.com/cdn/shop/articles/elution-order-630-1080_fa63e819-5a63-4bdd-8451-041cbce9a310_535x.jpg?v=1782971026)
![[Readers Insight] Why Mobile Phase Alterations Can Reverse Elution Order](http://www.welch-us.com/cdn/shop/articles/elution-order-630-1080_fa63e819-5a63-4bdd-8451-041cbce9a310_345x345@2x.jpg?v=1782971026)
![[Readers Insight] What Happens If Formic Acid Was Replaced by Acetic Acid in HPLC Analysis?](http://www.welch-us.com/cdn/shop/articles/acetic-acid-1080_535x.jpg?v=1781761748)
![[Readers Insight] What Happens If Formic Acid Was Replaced by Acetic Acid in HPLC Analysis?](http://www.welch-us.com/cdn/shop/articles/acetic-acid-1080_345x345@2x.jpg?v=1781761748)
![[Readers Insight] A Novel Analytical Approach: New Perspectives on Ionic Liquid Extraction](http://www.welch-us.com/cdn/shop/articles/1080-615_00fd03ec-e50b-4a24-ac8d-bb3f85408d1a_535x.jpg?v=1781595867)
![[Readers Insight] A Novel Analytical Approach: New Perspectives on Ionic Liquid Extraction](http://www.welch-us.com/cdn/shop/articles/1080-615_00fd03ec-e50b-4a24-ac8d-bb3f85408d1a_345x345@2x.jpg?v=1781595867)
