Knowledge Base

LP Columns: More Than Just Tolerating Strong Acids

December 31, 2025

Ultisil LP columns from Welch Materials are designed for low-pH chromatographic conditions, yet their advantages are more than simply resistance to strong acids. Read this article to learn how these columns outperform in a unique way.

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High-Resolution SEC-HPLC Aggregation Analysis of a Pembrolizumab Biosimilar Using Welch Xtimate™ SEC Columns

December 29, 2025

This application note presents a robust SEC-HPLC method for high-resolution aggregation analysis of a Pembrolizumab biosimilar using Welch Xtimate™ SEC-300 columns. The method demonstrates excellent separation of monomer, HMW aggregates, and LMW fragments, supporting routine QC and biosimilar comparability studies.

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[Readers Insight] Hydrophilic Interaction Chromatography (HILIC): A Nemesis to Polar Compounds

December 23, 2025

Polar compounds that refuse to stay on C18 columns can be frustrating. HILIC chromatography provides a versatile solution through partitioning and interaction mechanisms. In this post, we break down HILIC principles, column types, mobile phase strategies, and key method development tips to help you achieve reliable retention and separation.

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The Flow Cell in HPLC: A Small Component with Big Consequences

December 19, 2025

In LC analysis, column, mobile phase, and gradient are what we pay most attention to. However, sometimes the difference in flow cells can lead to significantly different results. This article explains the details behind it.

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[Readers Insight] Triethylamine as a Mobile Phase Additive: What Does It Do?

December 15, 2025

In this article, the author explains the uses of triethylamine as a mobile phase additive in HPLC, with real-world examples from pharmacopoeias and literatural applications.

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[Readers Insight] Why is Formic Acid Added to the Mobile Phase in Mass Spectrometry?

December 11, 2025

Formic acid is commonly seen added to the mobile phase in mass spectrometry methods, but have you wondered what specific role it plays, and can other acids substitute it? This article explains the role of formic acid in MS from the perspective of chemical reaction equilibrium thoroughly.

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Stop Flushing Arbitrarily! These Procedures Effectively Regenerates Your HPLC Columns

December 4, 2025

Inadequate column flushing causes HPLC columns to show abnormalities quickly even after only a few runs. In this article, we put together how to perform routine and regeneration flushing of Welch HPLC columns and store them correctly to extend their lives.

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[Readers Insight] Heterocycles Structural Analysis in HPLC Method Development

December 1, 2025

In this article, the author analyzes the structures of heterocycles (mostly aromatic heterocycles) and how the structures affect heterocycles' acid-base property.

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[Readers Insight] Choose Methanol or Acetonitrile? The Organic Mobile Phase in HPLC

November 17, 2025

In HPLC analysis, methanol and acetonitrile are the most common organic mobile phases. But have you ever thought about what exactly differentiates them? When should methanol be used and when acetonitrile? In this article, the author shares their thoughts on this matter.

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Understanding HPLC Pumps (Part II): Practical Maintenance

November 13, 2025

In the second part of this article, we explain the composition of an HPLC pump and the terminologies behind, as well as practical troubleshooting and preventive maintenance methods and schedules to pump failures.

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Understanding HPLC Pumps (Part I): Common Misconceptions

November 13, 2025

In the first part of this article, we explain common misconceptions of HPLC pumps, from the number of pump channels (and why they don't equal the number of pump heads or modules) to the differences between high- and low-pressure systems, and parallel and serial pumps.

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[Readers Insight] Why Do Substances with Similar Polarity Dissolve in Each Other?

November 12, 2025

In this article and attached video, the author discusses why substances with similar polarity dissolve in each other through dipole-dipole interactions, the energy change during dissolution, and the effect from entropy and Gibbs free energy.

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