Knowledge Base
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Best Practices for Mobile Phases in HPLC: Preparation, Use, and Storage
Proper preparation, handling, and storage of HPLC mobile phases are essential for achieving accurate, consistent results. This article outlines key considerations for selecting solvents, preparing mobile phases, and avoiding common pitfalls that can affect chromatographic performance and instrument longevity. -
HPLC Content Determination Method Validation: A Comprehensive Guide
This guide provides a detailed overview of HPLC method validation for content determination, highlighting key procedures, technical considerations, and common pitfalls to ensure accurate and reliable results. -
![[Readers Insight] Chromatographic Separation Strategies for Multi-Component Analysis](//www.welch-us.com/cdn/shop/articles/1080-multi-component_535x.jpg?v=1761629958)
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[Readers Insight] Chromatographic Separation Strategies for Multi-Component Analysis
Mass spectrometry simplifies multi-component analysis, but chromatographic separation remains essential for challenging cases. This article explores enhancements in gradient elution, column selection, temperature, flow rate, and mobile phase composition for separation efficiency and resolution. -
Gradient Optimization in Liquid Chromatography
In this article, we explore key considerations in gradient method development, and witness how optimizing gradient elution in HPLC is key to achieving efficient separation through a real-world case study. -
Key Concepts and Considerations of Preparative Liquid Chromatography
In this article, we discuss 10 essential aspects of preparative liquid chromatography, including definition, applications, workflow, key parameters, classification, difference from analytical LC, and practice. -
Unraveling the Case: Reasons Behind Poor LC RSD
A real-world case of poor reproducibility in LC analysis, with a general conclusion of common issues that cause RSD to be high. -
Issues and Solutions to the Use of Ion-Pairing Reagents
Explore the mechanism of ion-pairing reagents, and common issues and solutions when using ion-pairing chromatography. -
![[Reader Insight] Optimizing Matrix Curve Quantification in Mass Spectrometry](//www.welch-us.com/cdn/shop/articles/quantification-1080-1220_8f105584-f246-4674-94cb-cabd0eb003b4_535x.webp?v=1765518553)
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[Reader Insight] Optimizing Matrix Curve Quantification in Mass Spectrometry
In this article, the author explains matrix curve quantification in mass spectrometry, and offers strategies to minimize matrix effects and enhance accurate quantification. -
How to Distinguish Noise from Drift?
Learn how to identify and address baseline noise and drift in chromatography, understand their causes, and apply effective solutions for UV detectors. -
What is the Dead Volume of A Column?
This article introduces dead volume in HPLC: its definition, calculation methods, and tips to reduce it for better peak shapes and chromatographic efficiency. -
![[Reader Insight] Why Are Chromatograms](//www.welch-us.com/cdn/shop/articles/peak-shaped-1080_de59e5b5-d7c6-4b07-bbf9-0b17a516287b_535x.webp?v=1765509926)
![[Reader Insight] Why Are Chromatograms "Peak"-Shaped: A Guide for New Laboratory Analysts](//www.welch-us.com/cdn/shop/articles/peak-shaped-1080_de59e5b5-d7c6-4b07-bbf9-0b17a516287b_345x345@2x.webp?v=1765509926)
[Reader Insight] Why Are Chromatograms "Peak"-Shaped: A Guide for New Laboratory Analysts
Learn why chromatograms have the shape of peaks, understand their formation and what they reveal about analyte distribution and column efficiency.
![[Readers Insight] Chromatographic Separation Strategies for Multi-Component Analysis](http://www.welch-us.com/cdn/shop/articles/1080-multi-component_535x.jpg?v=1761629958)
![[Readers Insight] Chromatographic Separation Strategies for Multi-Component Analysis](http://www.welch-us.com/cdn/shop/articles/1080-multi-component_345x345@2x.jpg?v=1761629958)
![[Reader Insight] Optimizing Matrix Curve Quantification in Mass Spectrometry](http://www.welch-us.com/cdn/shop/articles/quantification-1080-1220_8f105584-f246-4674-94cb-cabd0eb003b4_535x.webp?v=1765518553)
![[Reader Insight] Optimizing Matrix Curve Quantification in Mass Spectrometry](http://www.welch-us.com/cdn/shop/articles/quantification-1080-1220_8f105584-f246-4674-94cb-cabd0eb003b4_345x345@2x.webp?v=1765518553)
![[Reader Insight] Why Are Chromatograms](http://www.welch-us.com/cdn/shop/articles/peak-shaped-1080_de59e5b5-d7c6-4b07-bbf9-0b17a516287b_535x.webp?v=1765509926)
![[Reader Insight] Why Are Chromatograms "Peak"-Shaped: A Guide for New Laboratory Analysts](http://www.welch-us.com/cdn/shop/articles/peak-shaped-1080_de59e5b5-d7c6-4b07-bbf9-0b17a516287b_345x345@2x.webp?v=1765509926)