This article explores how using Xtimate® Sugar-H and Sugar-Ca columns in series can significantly enhance resolution in sugar analysis. With real application cases in fermentation broth and pharmaceutical solutions, it demonstrates improved separation performance while maintaining safe pressure levels—offering a practical solution for challenging HPLC sugar separations.
Welch Xtimate Sugar Columns offer precise carbohydrate separation via ion coordination exchange, with easy regeneration and protection from metal ion interference.
Amino (NH2) columns excel in separating various sugars by hydrophilic interactions, while sugar columns use coordination exchange for better separation of monosaccharides and sugar alcohols.
