New Ultisil BHS & CHS Packing Materials for Peptides Are Now Available!
Welch Materials is proud to announce the official release of our latest high-performance chromatographic packing materials—Ultisil BHS and Ultisil CHS—specifically engineered for peptide purification. Built upon years of expertise and innovation in silica gel development, these new materials offer enhanced selectivity, high sample loading capacity, and outstanding scalability from lab-scale to industrial-scale purification.
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A Leap Forward in Silica Gel Technology
Both the BHS and CHS series are based on ultra-high purity spherical silica gel, featuring a larger effective specific surface area and a unique bonding process that significantly improves separation performance. These materials demonstrate excellent chemical and mechanical stability, making them ideal for rigorous peptide purification workflows. Whether you're optimizing yield, improving peak shape, or streamlining scale-up, Ultisil BHS and CHS provide the versatility and reliability you need.
Ultisil CHS Series
The Ultisil CHS series is designed to meet the evolving demands of modern peptide purification, especially for insulin, GLP-1, and its analogues. Featuring a highly deactivated surface and low metal residue, CHS delivers optimal performance in reducing non-specific adsorption and peak tailing. The precise particle and pore size distribution allow for higher recovery and better resolution in demanding separations.
- CHS C8: Offers moderate hydrophobic retention, ideal for general polypeptide and insulin purification.
- CHS C8 Pro: Optimized for synthetic GLP-1 and other peptide-based therapeutics.
| Ultisil CHS C8 | Ultisil CHS C8 Pro | |
| P/N | H02756-03600 | H02762-03600 |
| Bonded Phase | C8 | C8 |
| Particle Size (μm) | 10 | 10 |
| Pore Size (Å) | 100 | 100 |
| Max Pressure (MPa) | 40 | 40 |
| SSA (m2/g) | 320 | 320 |
| Carbon Load | 0.12 | 0.1 |
| pH | 2-9 | 2-10 |
Ultisil BHS Series
The BHS series is a robust, high-purity silica gel material built to accommodate a wide variety of small to medium-sized molecules, including peptides, antibiotics, and natural products. In reversed-phase chromatography, Ultisil BHS delivers well-balanced performance in separation capacity, loading capacity, recovery, and mechanical strength.
- BHS C18: General-purpose reversed-phase material suitable for most peptides, antibiotics, and small molecules. Available in 100Å and 120Å pore sizes.
- BHS C18 Plus: Slightly lower polarity than BHS C18, offering finer retention control.
- BHS C18 Pro: Specifically developed for purification of GLP-1 and fermentation-derived peptides.
Ultisil BHS C18 |
Ultisil BHS C18 Pro | ||
| P/N | H02755-03600 | H02755-03100 | H02761-17100 |
| Bonded Phase | C18 |
C18 | |
| Particle Size (μm) | 10 |
8 | |
| Pore Size (Å) | 100 | 120 | 120 |
| Max Pressure (MPa) | 40 |
40 | |
| SSA (m2/g) | 450 | 320 | 320 |
| Carbon Load | 19% |
15% | |
| pH | 2-9 |
2-10 | |
| Ultisil BHS C18 Plus | Ultisil BHS C8 | ||
| P/N | H02757-03100 | H02758-17100 | |
| Bonded Phase | C18 | C8 | |
| Particle Size (μm) | 10 | 8 | |
| Pore Size (Å) | 120 | 120 | |
| Max Pressure (MPa) | 40 | 40 | |
| SSA (m2/g) | 320 | 320 | |
| Carbon Load | 17% | 10% | |
| pH | 2-10 | 2-9 |
Note: Please contact us at info@welchmat.com to receive a quote.
Applications
1. Insulin Separation using Ultisil CHS C8
Insulin, a protein hormone, is secreted by beta cells in the islets of Langerhans in the pancreas in response to various endogenous or exogenous stimuli, such as glucose, lactose, nucleic acids, arginine, and glucagon. While insulin is the only hormone capable of lowering blood sugar levels and promoting the synthesis of glycogen, fat, and protein, its exogenous form is primarily utilized for diabetes treatment. Currently, insulin purification predominantly relies on reversed-phase chromatography, with Ultisil CHS C8 (10 μm, 100 Å) packing material demonstrating exceptional efficacy in insulin separation.
| Packing Material | Ultisil CHS C8 (10 μm, 100 Å) |
| Sample | Insulin |
| Loading Capacity | 1% |
| Flow Rate | 3 mL/min |
| Purity | 99.60% |
| Recovery | 88.50% |
2. Antibiotic—Teicoplanin Separation using Ultisil BHS C18
Teicoplanin, also known as Teichomycin, is a potent antibiotic used to treat Gram-positive bacterial infections caused by both anaerobic and aerobic bacteria. It is more effective than vancomycin and has fewer adverse reactions.
| Packing Material | Ultisil BHS C18 (10 μm, 100 Å) |
| Sample | Teicoplanin |
| Loading Capacity | 3% |
| Flow Rate | 3 mL/min |
| Purity | 99.50% |
| Recovery | 80.02% |
3. Chemical Synthesis of GLP-1 Liraglutide using Ultisil CHS C8 Pro
Ultisil CHS C8 Pro is a high-performance silica gel chromatographic packing material specifically designed for the purification and production of chemically synthesized GLP-1. Built upon the strengths of the Ultisil CHS series, it features uniform particle size and an exceptionally high effective specific surface area. Additionally, its innovative bonding technology enhances separation efficiency, significantly improving product purity, recovery rates, and loading capacity.
| Packing Material | Ultisil CHS C8 Pro (10 μm, 100 Å) |
| Sample | Liraglutide |
| Loading Capacity | 1% |
| Flow Rate | 3 mL/min |
| Purity | 99.77% |
| Recovery | 74.79% |
4. Chemical Synthesis of GLP-1 Tirzepatide
Tirzepatide is a novel dual agonist of the glucose-dependent insulinotropic polypeptide (GIP) receptor and glucagon-like peptide-1 (GLP-1) receptor, designed to bind to albumin and extend its half-life. Tirzepatide enhances both phase I and phase II insulin secretion while reducing glucagon levels in a glucose-dependent manner. It also lowers fasting and postprandial glucose concentrations, decreases food intake, and promotes weight loss in patients with type 2 diabetes mellitus.
Using Ultisil CHS C8 Pro
| Packing Material | Ultisil CHS C8 Pro (10 μm, 100 Å) |
| Sample | Tirzepatide |
| Loading Capacity | 1% |
| Flow Rate | 3 mL/min |
| Purity | 99.73% |
| Recovery | 72.90% |
Using Ultisil BHS C18 Plus
| Packing Material | Ultisil BHS C18 Plus (8 μm, 120 Å) | |
| Sample | Tirzepatide | |
| Loading Capacity | 1% | |
| Flow Rate | 3 mL/min | |
| Purity | 90.00% | 99.11% |
| Recovery | 74.89% | 87.87% |
5. Fermentation synthesis of GLP-1 Semaglutide
Semaglutide is an anti-diabetic medication used for the treatment of type 2 diabetes and an anti-obesity medication used for long-term weight management. It is a peptide similar to the hormone glucagon-like peptide-1 (GLP-1), modified with a side chain.
Ultisil CHS C8 Pro and Ultisil BHS C18 Pro are designed for the purification of GLP-1 during fermentation synthesis. They effectively separate various biological impurities that arise during the synthesis process. With very high sample loading capacities and recovery rates, they ensure the purity of the final product while significantly enhancing production efficiency.
Using Ultisil CHS C8 Pro
| Packing Material | Ultisil CHS C8 Pro (10 μm, 100 Å) |
|
| Sample | Semaglutide | |
| Loading Capacity | 240 mg | |
| Flow Rate | 3 mL/min | |
| Purity | 96.67% | 99.86% |
| Recovery | 75.68% | 86.68% |
Using Ultisil BHS C18 Pro
| Packing Material | Ultisil BHS C18 Pro (8 μm, 100 Å) | |
| Sample | Semaglutide | |
| Loading Capacity | 2% | |
| Flow Rate | 3 mL/min | |
| Purity | 90.00% | 99.81% |
| Recovery | 93.00% | 85.00% |
| Overall Recovery | 79.05% | |
6. Oligopeptides purification using Ultisil BHS C18 Pro
Ultisil BHS C18 Pro is also a specialized silica gel packing material designed for the efficient separation of oligopeptides. By utilizing advanced bonding and anti-tailing technologies, it significantly improves separation efficiency and recovery rates.
| Packing Material | Ultisil BHS C18 Pro (8 μm, 120 Å) |
| Sample | Oligopeptide-5 |
| Loading Capacity | 1% |
| Flow Rate | 3 mL/min |
| Purity | 99.00% |
| Recovery | 90.02% |
Conclusion
Whether you're conducting initial process development or running full-scale production, Ultisil BHS and CHS provide a powerful foundation for efficient, reliable, and scalable peptide purification. Contact Welch Materials today at info@welchmat.com to request a quote or learn more about how these new solutions can enhance your workflows.
