Knowledge Base

The existence of hydrogen bonding in acetonitrile-water solutions is a fundamental question. In this article, we will discuss the question in details, including the physicochemical properties of acetonitrile and water, the interaction that takes place when acetonitrile is dissolved in water, and the abnormalities that may occur when using acetonitrile-water solutions as mobile phase or for sample extraction.

In HPLC analysis, methanol and acetonitrile are the most common organic mobile phases. But have you ever thought about what exactly differentiates them? When should methanol be used and when acetonitrile? In this article, the author shares their thoughts on this matter.