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Knowledge Base

[Readers Insight] How to Analyze Fragment Ions in Mass Spectrometry?

January 19, 2026

In this article, we investigate the fundamental mechanisms of ion fragmentation within mass spectrometry workflows and explore why systematically mapping fragment-ion structures is essential for deeper mass-spectrometric interpretation. It explores the relationship between electronic displacement and dissociation pathways, emphasizing structural reconstruction over simple pattern recognition.

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[Readers Insight] Asking the Questions Right: A Guide to Troubleshooting Consultations in HPLC Method Development

January 15, 2026

Mastering HPLC method development requires more than technical skill; it demands effective communication. This guide explores how junior scientists can overcome "question anxiety" by utilizing precise data packages and structured inquiries. Learn to transform troubleshooting consultations into professional growth opportunities, ensuring faster resolutions and building lasting laboratory expertise.

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[Readers Insight] The Interplay between Column Properties and Peak Integration

January 14, 2026

In HPLC, the relationship between physical separation hardware and mathematical data processing is often overlooked. This article explores the inconspicuous but critical connection between column properties and the fidelity of peak area calculation, examining why theoretical constancy in integration often fails to align with empirical laboratory observations.

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[Readers Insight] Gradient vs. Isocratic Elution: Which to Choose?

January 6, 2026

In this article, the author evaluates isocratic and gradient elution strategies in HPLC method development, providing expert guidance on selecting elution modes for pharmaceutical assays, impurity profiling, and complex separations.

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[Readers Insight] Hydrophilic Interaction Chromatography (HILIC): A Nemesis to Polar Compounds

December 23, 2025

Polar compounds that refuse to stay on C18 columns can be frustrating. HILIC chromatography provides a versatile solution through partitioning and interaction mechanisms. In this post, we break down HILIC principles, column types, mobile phase strategies, and key method development tips to help you achieve reliable retention and separation.

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[Readers Insight] Triethylamine as a Mobile Phase Additive: What Does It Do?

December 15, 2025

In this article, the author explains the uses of triethylamine as a mobile phase additive in HPLC, with real-world examples from pharmacopoeias and literatural applications.

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[Readers Insight] Why is Formic Acid Added to the Mobile Phase in Mass Spectrometry?

December 11, 2025

Formic acid is commonly seen added to the mobile phase in mass spectrometry methods, but have you wondered what specific role it plays, and can other acids substitute it? This article explains the role of formic acid in MS from the perspective of chemical reaction equilibrium thoroughly.

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[Readers Insight] Heterocycles Structural Analysis in HPLC Method Development

December 1, 2025

In this article, the author analyzes the structures of heterocycles (mostly aromatic heterocycles) and how the structures affect heterocycles' acid-base property.

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[Readers Insight] Choose Methanol or Acetonitrile? The Organic Mobile Phase in HPLC

November 17, 2025

In HPLC analysis, methanol and acetonitrile are the most common organic mobile phases. But have you ever thought about what exactly differentiates them? When should methanol be used and when acetonitrile? In this article, the author shares their thoughts on this matter.

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[Readers Insight] Why Do Substances with Similar Polarity Dissolve in Each Other?

November 12, 2025

In this article and attached video, the author discusses why substances with similar polarity dissolve in each other through dipole-dipole interactions, the energy change during dissolution, and the effect from entropy and Gibbs free energy.

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[Readers Insight] HPLC Column Selection: Core to Method Development (Part II)

November 5, 2025

In this article, the author shares their answers for 8 common questions in column selection, aiming to guide users in choosing the most suitable column through practical experimentation.

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[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)

November 5, 2025

A chromatographic column is the heart of separation. However, with thousands of column options on the market, it is often asked how to choose "the best one". In this article, the author explains the classifications of HPLC columns and how to select the most cmpatible column for an analyte.

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