Knowledge Base
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![[Readers Insight] Asking the Questions Right: A Guide to Troubleshooting Consultations in HPLC Method Development](//www.welch-us.com/cdn/shop/articles/asking-115-1080_e1af459a-8c94-4cee-a7ea-1d8f32e355df_535x.jpg?v=1768532002)
![[Readers Insight] Asking the Questions Right: A Guide to Troubleshooting Consultations in HPLC Method Development](//www.welch-us.com/cdn/shop/articles/asking-115-1080_e1af459a-8c94-4cee-a7ea-1d8f32e355df_345x345@2x.jpg?v=1768532002)
[Readers Insight] Asking the Questions Right: A Guide to Troubleshooting Consultations in HPLC Method Development
Mastering HPLC method development requires more than technical skill; it demands effective communication. This guide explores how junior scientists can overcome "question anxiety" by utilizing precise data packages and structured inquiries. Learn to transform troubleshooting consultations into professional growth opportunities, ensuring faster resolutions and building lasting laboratory expertise. -
LP Columns: More Than Just Tolerating Strong Acids
Ultisil LP columns from Welch Materials are designed for low-pH chromatographic conditions, yet their advantages are more than simply resistance to strong acids. Read this article to learn how these columns outperform in a unique way. -
![[Readers Insight] Hydrophilic Interaction Chromatography (HILIC): A Nemesis to Polar Compounds](//www.welch-us.com/cdn/shop/articles/1080_a134ed46-3aa6-4c67-878d-1c3786a088e9_535x.jpg?v=1767751595)
![[Readers Insight] Hydrophilic Interaction Chromatography (HILIC): A Nemesis to Polar Compounds](//www.welch-us.com/cdn/shop/articles/1080_a134ed46-3aa6-4c67-878d-1c3786a088e9_345x345@2x.jpg?v=1767751595)
[Readers Insight] Hydrophilic Interaction Chromatography (HILIC): A Nemesis to Polar Compounds
Polar compounds that refuse to stay on C18 columns can be frustrating. HILIC chromatography provides a versatile solution through partitioning and interaction mechanisms. In this post, we break down HILIC principles, column types, mobile phase strategies, and key method development tips to help you achieve reliable retention and separation. -
![[Readers Insight] Triethylamine as a Mobile Phase Additive: What Does It Do?](//www.welch-us.com/cdn/shop/articles/triethylamine-1080-1215_c5628b48-1386-4303-9888-40c9ff3ebe24_535x.jpg?v=1767518636)
![[Readers Insight] Triethylamine as a Mobile Phase Additive: What Does It Do?](//www.welch-us.com/cdn/shop/articles/triethylamine-1080-1215_c5628b48-1386-4303-9888-40c9ff3ebe24_345x345@2x.jpg?v=1767518636)
[Readers Insight] Triethylamine as a Mobile Phase Additive: What Does It Do?
In this article, the author explains the uses of triethylamine as a mobile phase additive in HPLC, with real-world examples from pharmacopoeias and literatural applications. -
![[Readers Insight] Heterocycles Structural Analysis in HPLC Method Development](//www.welch-us.com/cdn/shop/articles/heterocycles_0d7b25a5-0c26-4e1f-bf88-6892aee70724_535x.jpg?v=1766539886)
![[Readers Insight] Heterocycles Structural Analysis in HPLC Method Development](//www.welch-us.com/cdn/shop/articles/heterocycles_0d7b25a5-0c26-4e1f-bf88-6892aee70724_345x345@2x.jpg?v=1766539886)
[Readers Insight] Heterocycles Structural Analysis in HPLC Method Development
In this article, the author analyzes the structures of heterocycles (mostly aromatic heterocycles) and how the structures affect heterocycles' acid-base property. -
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part II)](//www.welch-us.com/cdn/shop/articles/1080-1105-2_b4aa3b37-ea3f-4e79-a57a-c6d3b30d6233_535x.jpg?v=1765505696)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part II)](//www.welch-us.com/cdn/shop/articles/1080-1105-2_b4aa3b37-ea3f-4e79-a57a-c6d3b30d6233_345x345@2x.jpg?v=1765505696)
[Readers Insight] HPLC Column Selection: Core to Method Development (Part II)
In this article, the author shares their answers for 8 common questions in column selection, aiming to guide users in choosing the most suitable column through practical experimentation. -
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)](//www.welch-us.com/cdn/shop/articles/columnselection2-1080-1105_723f3401-a432-4f3b-af91-ea4881d85d16_535x.jpg?v=1765519204)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)](//www.welch-us.com/cdn/shop/articles/columnselection2-1080-1105_723f3401-a432-4f3b-af91-ea4881d85d16_345x345@2x.jpg?v=1765519204)
[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)
A chromatographic column is the heart of separation. However, with thousands of column options on the market, it is often asked how to choose "the best one". In this article, the author explains the classifications of HPLC columns and how to select the most cmpatible column for an analyte. -
![[Readers Insight] Mobile Phase Selection in Method Development: How to Optimize](//www.welch-us.com/cdn/shop/articles/mpoptimize-1080-1020_535x.jpg?v=1762394840)
![[Readers Insight] Mobile Phase Selection in Method Development: How to Optimize](//www.welch-us.com/cdn/shop/articles/mpoptimize-1080-1020_345x345@2x.jpg?v=1762394840)
[Readers Insight] Mobile Phase Selection in Method Development: How to Optimize
In method development, the selection of mobile phases is an important part to success. This article discusses thoroughly different mobile phases and their use in HPLC and LC-MS, and provides recommendations for initial mobile phase choices.
![[Readers Insight] Asking the Questions Right: A Guide to Troubleshooting Consultations in HPLC Method Development](http://www.welch-us.com/cdn/shop/articles/asking-115-1080_e1af459a-8c94-4cee-a7ea-1d8f32e355df_535x.jpg?v=1768532002)
![[Readers Insight] Asking the Questions Right: A Guide to Troubleshooting Consultations in HPLC Method Development](http://www.welch-us.com/cdn/shop/articles/asking-115-1080_e1af459a-8c94-4cee-a7ea-1d8f32e355df_345x345@2x.jpg?v=1768532002)
![[Readers Insight] Hydrophilic Interaction Chromatography (HILIC): A Nemesis to Polar Compounds](http://www.welch-us.com/cdn/shop/articles/1080_a134ed46-3aa6-4c67-878d-1c3786a088e9_535x.jpg?v=1767751595)
![[Readers Insight] Hydrophilic Interaction Chromatography (HILIC): A Nemesis to Polar Compounds](http://www.welch-us.com/cdn/shop/articles/1080_a134ed46-3aa6-4c67-878d-1c3786a088e9_345x345@2x.jpg?v=1767751595)
![[Readers Insight] Triethylamine as a Mobile Phase Additive: What Does It Do?](http://www.welch-us.com/cdn/shop/articles/triethylamine-1080-1215_c5628b48-1386-4303-9888-40c9ff3ebe24_535x.jpg?v=1767518636)
![[Readers Insight] Triethylamine as a Mobile Phase Additive: What Does It Do?](http://www.welch-us.com/cdn/shop/articles/triethylamine-1080-1215_c5628b48-1386-4303-9888-40c9ff3ebe24_345x345@2x.jpg?v=1767518636)
![[Readers Insight] Heterocycles Structural Analysis in HPLC Method Development](http://www.welch-us.com/cdn/shop/articles/heterocycles_0d7b25a5-0c26-4e1f-bf88-6892aee70724_535x.jpg?v=1766539886)
![[Readers Insight] Heterocycles Structural Analysis in HPLC Method Development](http://www.welch-us.com/cdn/shop/articles/heterocycles_0d7b25a5-0c26-4e1f-bf88-6892aee70724_345x345@2x.jpg?v=1766539886)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part II)](http://www.welch-us.com/cdn/shop/articles/1080-1105-2_b4aa3b37-ea3f-4e79-a57a-c6d3b30d6233_535x.jpg?v=1765505696)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part II)](http://www.welch-us.com/cdn/shop/articles/1080-1105-2_b4aa3b37-ea3f-4e79-a57a-c6d3b30d6233_345x345@2x.jpg?v=1765505696)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)](http://www.welch-us.com/cdn/shop/articles/columnselection2-1080-1105_723f3401-a432-4f3b-af91-ea4881d85d16_535x.jpg?v=1765519204)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)](http://www.welch-us.com/cdn/shop/articles/columnselection2-1080-1105_723f3401-a432-4f3b-af91-ea4881d85d16_345x345@2x.jpg?v=1765519204)
![[Readers Insight] Mobile Phase Selection in Method Development: How to Optimize](http://www.welch-us.com/cdn/shop/articles/mpoptimize-1080-1020_535x.jpg?v=1762394840)
![[Readers Insight] Mobile Phase Selection in Method Development: How to Optimize](http://www.welch-us.com/cdn/shop/articles/mpoptimize-1080-1020_345x345@2x.jpg?v=1762394840)