In this article, the author shares their answers for 8 common questions in column selection, aiming to guide users in choosing the most suitable column through practical experimentation.
A chromatographic column is the heart of separation. However, with thousands of column options on the market, it is often asked how to choose "the best one". In this article, the author explains the classifications of HPLC columns and how to select the most cmpatible column for an analyte.
In method development, the selection of mobile phases is an important part to success. This article discusses thoroughly different mobile phases and their use in HPLC and LC-MS, and provides recommendations for initial mobile phase choices.
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![[Readers Insight] HPLC Column Selection: Core to Method Development (Part II)](http://www.welch-us.com/cdn/shop/articles/1080-1105-2_535x.jpg?v=1762395398)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part II)](http://www.welch-us.com/cdn/shop/articles/1080-1105-2_345x345@2x.jpg?v=1762395398)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)](http://www.welch-us.com/cdn/shop/articles/columnselection2-1080-1105_535x.jpg?v=1762395940)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)](http://www.welch-us.com/cdn/shop/articles/columnselection2-1080-1105_345x345@2x.jpg?v=1762395940)
![[Readers Insight] Mobile Phase Selection in Method Development: How to Optimize](http://www.welch-us.com/cdn/shop/articles/mpoptimize-1080-1020_535x.jpg?v=1762394840)
![[Readers Insight] Mobile Phase Selection in Method Development: How to Optimize](http://www.welch-us.com/cdn/shop/articles/mpoptimize-1080-1020_345x345@2x.jpg?v=1762394840)