Knowledge Base
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Advanced Chromatographic Resolution of Chiral Compounds, Part III: Alcohols and Diols
Alcohols and diols are among the most common functionalized intermediates in organic synthesis and pharmaceutical development, and chirality can influence their efficacy greatly. In this article, we introduce applications on the chiral separation of several typical alcohols and diol using Blossmate chiral columns. -
Mitigating Non-Specific Adsorption in Size Exclusion Chromatography: The Welch Xtimate Bio SEC Solution
Biopharmaceutical integrity relies on precise molecular size characterization. In this article, we explain how Welch Xtimate Bio SEC columns mitigate non-specific adsorption in SEC compared with competitors, and provide guides on optimizing mobile phase in different SEC assays. -
Advanced Chromatographic Resolution of Chiral Compounds, Part II: Amino Acids
Amino acids and their derivatives serve as fundamental building blocks in the synthesis of bioactive peptides and chiral drugs, and their chirality greatly affect the quality of the final product. In this article, we introduce the enantiomer separation of four different amino acid derivatives with Welch Blossmate chiral columns. -
Advanced Chromatographic Resolution of Chiral Compounds, Part I: NSAIDs
Achieving enantiomeric purity is vital for the clinical safety and efficacy. Among the most widely prescribed analgesics, NSAIDs presents a persistent analytical challenge due to the subtle structural variations between their R- and S-enantiomers. In this article, we present the chiral separation of three significant "profen" NSAIDs (flurbiprofen, tiaprofenic acid, and carprofen) with Welch's new Blossmate chiral columns. -
Achieving Superior Isomer Separation with Welch Materials Columns
The separation of isomers is often a key challenge in HPLC due to its differences being so subtle that standard columns fail to resolve. In this article, we introduce three Welch Materials columns that perform well in isomer separation. -
Precision Characterization of Recombinant Humanized Type III Collagen: Navigating Analytical Challenges with Xtimate Bio SEC
In this article, we explore the analytical challenges of recombinant humanized type III collagen and show how Xtimate Bio SEC columns support accurate purity and aggregate profiling. The article also highlights the role of SEC in quality control and demonstrates strong chromatographic performance for collagen characterization. -
The Critical Role of Chiral Separation and Its Modern Solutions
Chirality can mean the difference between medicine and catastrophe. In this article, we summarize the necessity of enantiomeric precision and explain how our Blossmate chiral columns offer flexible, MS-compatible solutions for analytical and preparative workflows, with chromatograms comparing our and competitor's column performances. -
Advancing Metabolic Therapeutics: An Overview of Retatrutide and the Analytical Rigor of Peptide Quantification
Retatrutide is a new synthetic peptide that facilitates weight loss through a dual-action mechanism. In this article, we utilize an Ultisil LP-C18 column for the determination of Retatrutide that achieves baseline separation with high resolution records. -
LP Columns: More Than Just Tolerating Strong Acids
Ultisil LP columns from Welch Materials are designed for low-pH chromatographic conditions, yet their advantages are more than simply resistance to strong acids. Read this article to learn how these columns outperform in a unique way. -
High-Resolution SEC-HPLC Aggregation Analysis of a Pembrolizumab Biosimilar Using Welch Xtimate™ SEC Columns
This application note presents a robust SEC-HPLC method for high-resolution aggregation analysis of a Pembrolizumab biosimilar using Welch Xtimate™ SEC-300 columns. The method demonstrates excellent separation of monomer, HMW aggregates, and LMW fragments, supporting routine QC and biosimilar comparability studies. -
A Premier Alkaloid: Nicotine and the Strategy for Developing Its Determination Method
Nicotine analysis is notoriously challenging due to its weak retention on conventional columns. This article shares a practical UHPLC-MS method using the Welch Ultisil HILIC Amphion II column, detailing the development approach, chromatographic conditions, and key advantages for reliable nicotine detection. -
Determination of Mannitol Related Substances with Xtimate Sugar-Ca
Mannitol is a substance with important applications in pharmaceutical and food. See how Welch Xtimate Sugar-Ca columns perform well in determination of mannitol related substances in accordance with Chinese Pharmacopoeia 2025 Edition.
