Nicotine analysis is notoriously challenging due to its weak retention on conventional columns. This article shares a practical UHPLC-MS method using the Welch Ultisil HILIC Amphion II column, detailing the development approach, chromatographic conditions, and key advantages for reliable nicotine detection.
Mannitol is a substance with important applications in pharmaceutical and food. See how Welch Xtimate Sugar-Ca columns perform well in determination of mannitol related substances in accordance with Chinese Pharmacopoeia 2025 Edition.
This article explores how using Xtimate® Sugar-H and Sugar-Ca columns in series can significantly enhance resolution in sugar analysis. With real application cases in fermentation broth and pharmaceutical solutions, it demonstrates improved separation performance while maintaining safe pressure levels—offering a practical solution for challenging HPLC sugar separations.
