Nicotine analysis is notoriously challenging due to its weak retention on conventional columns. This article shares a practical UHPLC-MS method using the Welch Ultisil HILIC Amphion II column, detailing the development approach, chromatographic conditions, and key advantages for reliable nicotine detection.
Mannitol is a substance with important applications in pharmaceutical and food. See how Welch Xtimate Sugar-Ca columns perform well in determination of mannitol related substances in accordance with Chinese Pharmacopoeia 2025 Edition.
This article explores how using Xtimate® Sugar-H and Sugar-Ca columns in series can significantly enhance resolution in sugar analysis. With real application cases in fermentation broth and pharmaceutical solutions, it demonstrates improved separation performance while maintaining safe pressure levels—offering a practical solution for challenging HPLC sugar separations.
In this article, we explore key considerations in gradient method development, and witness how optimizing gradient elution in HPLC is key to achieving efficient separation through a real-world case study.
