Peak area is the preferred metric for quantification in chromatography due to its consistency across variable peak shapes and resistance to signal distortion. Unlike peak height or volume, it offers reliable integration even with minor peak deformation or interference, making it the most robust choice in routine analysis.
This guide provides a detailed overview of HPLC method validation for content determination, highlighting key procedures, technical considerations, and common pitfalls to ensure accurate and reliable results.
In this article, the author explains matrix curve quantification in mass spectrometry, and offers strategies to minimize matrix effects and enhance accurate quantification.
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