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Introducing Advanchrom C18: Redefining HPLC Performance Through Innovation

Introducing Advanchrom C18: Redefining HPLC Performance Through Innovation

Table of contents
Introduction Breakthrough in Core Technology Key Advantages of Advanchrom C18 1. Wide pH Tolerance 2. Enhanced Anti-Fouling Properties 3. Exceptional Batch-to-Batch Reproducibility Rigorously Validated Reproducibility Proven High-Temperature Endurance Superior High-pH Stability Conclusion Appendix. Product Specifications

Introduction

Welch Materials is proud to unveil its latest innovation—Advanchrom C18, a high-performance HPLC column meticulously engineered for demanding separation and analysis workflows. Designed to deliver exceptional reliability and precision, Advanchrom C18 sets a new benchmark in chromatographic performance.

Advanchrom C18

Breakthrough in Core Technology

Built upon ultra-pure hybrid silica, Advanchrom C18 enables an extended pH operating range of 1 to 12.5, far surpassing conventional industry standards. Coupled with our unique bonding and end-capping processes, the column maintains outstanding separation performance even under extreme analytical conditions.

Key Advantages of Advanchrom C18

1. Wide pH Tolerance

Capable of withstanding mobile phases across pH 1–12.5, this column is particularly well-suited for the separation of basic compounds in complex matrices. It offers flexibility for method development and expands application possibilities in challenging alkaline conditions.

2. Enhanced Anti-Fouling Properties

Advanchrom C18 significantly reduces the adsorption of non-polar compounds, minimizing impurity retention and column contamination. In scenarios that require high column efficiency, this column reduces maintenance downtime caused by column contamination, greatly improving overall lab efficiency. Ideal for high-throughput testing scenarios such as:

  • Food safety analysis (e.g., preservatives and additives)
  • Traditional Chinese medicine (e.g., crude herbs and decoction pieces)
  • Pharmaceutical QC (e.g., reaction monitoring and impurity profiling)

3. Exceptional Batch-to-Batch Reproducibility

Thanks to a fully standardized manufacturing process and rigorous quality control, Advanchrom C18 ensures consistency in key performance metrics—retention time, resolution, and plate number—across production batches. This consistency supports seamless method transfer and reliable large-scale testing.

Advanchrom C18

Rigorously Validated Reproducibility

Chromatographic Conditions:

  • Column Specification: 4.6×150 mm, 5 µm
  • Mobile Phase: acetonitrile/water = 65/35
  • Flow Rate: 1.0 mL/min

Analytes:

  • Uracil
  • Phenol
  • 4-Nitrochlorobenzene
  • Toluene
Chromatogram of reproducibility test
Batch # Ret. Time Plates Tailing Resolution
1 4.574 11346 1.09 4.47
2 4.612 11397 1.10 4.43
3 4.603 10921 1.07 4.39
4 4.589 11320 1.08 4.40
5 4.583 11453 1.08 4.38

Five batches of column packing material were validated to deliver consistent retention time, theoretical plates, tailing factors, and resolution, allowing analysts to proceed with confidence, knowing batch variation will not compromise their results.

Proven High-Temperature Endurance

Chromatographic Conditions:

  • Column Specification: 4.6×250 mm, 5 µm
  • Mobile Phase: acetonitrile/water = 65/35
  • Flow Rate: 1.0 mL/min
  • Column Temperature: 65 ℃

Analytes:

  • Uracil
  • Phenol
  • 4-Nitrochlorobenzene
  • Toluene
Chromatogram of temperature endurance test

Subjected to 65 °C column temperature and 1,000 consecutive injections, Advanchrom C18 demonstrated excellent peak shape and maintained high theoretical plate counts. Even under prolonged high-temperature use, the column performs with remarkable stability, minimizing method interruption and reruns.

Superior High-pH Stability

Chromatographic Conditions:

  • Column Specification: 2.1×50 mm, 5 µm
  • Mobile Phase: 10 mmol/L ammonium carbonate/methanol = 70/30 (pH=10.0)
  • Flow Rate: 0.21 mL/min
  • Column Temperature: 50 ℃
  • Injection Volume: 0.6 µL
  • Wavelength: 254 nm

Analytes:

  • Uracil
  • Sodium p-toluenesulfonate
  • Acetanilide
  • Procainamide
Chromatogram of Advanchrom C18 in high pH conditions
Advanchrom C18
Chromatogram of conventional C18 in high pH conditions
Conventional C18, note the peak fronting at 60 hours

After 60 hours of continuous injection under high-pH mobile phase conditions, Advanchrom C18 continued to deliver sharp peaks and high efficiency. Its robust resistance to alkaline degradation ensures reliable long-term performance in harsh conditions.

Conclusion

Advanchrom C18 embodies Welch’s commitment to advancing chromatographic technology. With its cutting-edge design and superior durability, it empowers laboratories in food safety, pharmaceutical development, and chemical analysis to achieve higher efficiency, greater accuracy, and reduced operational costs.

Step into the future of HPLC with Advanchrom C18—where innovation drives precision.

Advanchrom C18

Appendix: Product Specifications

Stationary Phase Particle Size pH Range Carbon Load Max. Temperature
C18 5 µm 1-12.5 14% 60 ℃