Formic acid is commonly seen added to the mobile phase in mass spectrometry methods, but have you wondered what specific role it plays, and can other acids substitute it? This article explains the role of formic acid in MS from the perspective of chemical reaction equilibrium thoroughly.
In HPLC analysis, methanol and acetonitrile are the most common organic mobile phases. But have you ever thought about what exactly differentiates them? When should methanol be used and when acetonitrile? In this article, the author shares their thoughts on this matter.
Proper preparation, handling, and storage of HPLC mobile phases are essential for achieving accurate, consistent results. This article outlines key considerations for selecting solvents, preparing mobile phases, and avoiding common pitfalls that can affect chromatographic performance and instrument longevity.
![[Readers Insight] Why is Formic Acid Added to the Mobile Phase in Mass Spectrometry?](//www.welch-us.com/cdn/shop/articles/formic-acid-1080_285b53f7-6c39-44e1-98eb-566f7e73016d_535x.jpg?v=1765503777)
![[Readers Insight] Why is Formic Acid Added to the Mobile Phase in Mass Spectrometry?](//www.welch-us.com/cdn/shop/articles/formic-acid-1080_285b53f7-6c39-44e1-98eb-566f7e73016d_345x345@2x.jpg?v=1765503777)
![[Readers Insight] Choose Methanol or Acetonitrile? The Organic Mobile Phase in HPLC](//www.welch-us.com/cdn/shop/articles/1080_746151f5-d530-4722-b64b-4cc9cff7a1d3_535x.jpg?v=1765506053)
![[Readers Insight] Choose Methanol or Acetonitrile? The Organic Mobile Phase in HPLC](//www.welch-us.com/cdn/shop/articles/1080_746151f5-d530-4722-b64b-4cc9cff7a1d3_345x345@2x.jpg?v=1765506053)
![[Readers Insight] Why is Formic Acid Added to the Mobile Phase in Mass Spectrometry?](http://www.welch-us.com/cdn/shop/articles/formic-acid-1080_285b53f7-6c39-44e1-98eb-566f7e73016d_535x.jpg?v=1765503777)
![[Readers Insight] Why is Formic Acid Added to the Mobile Phase in Mass Spectrometry?](http://www.welch-us.com/cdn/shop/articles/formic-acid-1080_285b53f7-6c39-44e1-98eb-566f7e73016d_345x345@2x.jpg?v=1765503777)
![[Readers Insight] Choose Methanol or Acetonitrile? The Organic Mobile Phase in HPLC](http://www.welch-us.com/cdn/shop/articles/1080_746151f5-d530-4722-b64b-4cc9cff7a1d3_535x.jpg?v=1765506053)
![[Readers Insight] Choose Methanol or Acetonitrile? The Organic Mobile Phase in HPLC](http://www.welch-us.com/cdn/shop/articles/1080_746151f5-d530-4722-b64b-4cc9cff7a1d3_345x345@2x.jpg?v=1765506053)
