Knowledge Base

[Readers Insight] Are You Choosing the Right Quantifier Ion for Mass Spectrometry?

August 20, 2025

In this article, the author discusses the principles behind selecting quantifier ions in triple quadrupole mass spectrometry, with a case study to illustrate how ion choice impacts sensitivity and accuracy.

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[Readers Insight] Why Do M-Shaped Peaks Occur?

August 14, 2025

M-shaped peaks can puzzle even experienced chromatographers, appearing unexpectedly and resisting simple fixes. This article draws on expert insights to explore their possible origins and practical approaches, guiding analysts toward more reliable and consistent chromatographic results.

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Agarose: A Versatile Packing Material for Protein Purification

August 5, 2025

Agarose-based chromatographic media offer a compelling blend of biocompatibility, versatility, and affordability. By mastering the principles of agarose microsphere preparation and crosslinking, chromatographers can tailor supports to a vast array of protein purification challenges, ensuring high yield, purity, and process robustness.

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Mastering Gas Chromatography in 7 Comprehensive Steps!

July 28, 2025

This article guides analysts in GC analyses through efficient sample preparation, instrument configuration, initial condition setup, separation optimization, qualitative identification using retention indices or GC–MS, quantitative analysis via internal standards or standard addition, rigorous method validation, ensuring accurate, precise, reproducible results across diverse unknown sample analyses consistently.

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Choosing Gas Chromatography Columns: Key Parameters

July 24, 2025

In gas chromatography, column length, internal diameter, and film thickness critically determine efficiency, retention times, pressure requirements, and sample capacity. This article reviews how each parameter impacts chromatographic performance and provides practical selection guidelines—balancing resolution, analysis time, and cost—to help analysts choose optimal capillary columns for diverse applications.

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Uncovering Overlooked Factors Behind Abnormal Baselines

July 23, 2025

A stable, flat baseline is essential for accurate HPLC analyses, yet analysts may grapple with noise and drift. This article explores key baseline disturbances—sawtooth patterns, pulsations, chaotic fluctuations, and drift—pinpoints their root causes, and offers precise remedies. A real-world case study demonstrates systematic troubleshooting to restore baseline integrity.

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[Readers Insight] How to Quickly Establish Robust HPLC Analytical Methods: A 5-Step Expert Guide

May 8, 2025

This practical article guides new chromatographers through five essential steps to build robust HPLC methods: structural analysis, solvent selection, signal enhancement, elution optimization, and method validation.

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Pursuing Excellence in Pharmaceutical Quality Control: Best Practices and Insights for Laboratories

April 29, 2025

In this article, one of Welch’s long-term customer shares their practices and insights on pharmaceutical laboratory quality control in both HPLC and GC, along with their firsthand experience of Welch’s HPLC columns.

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[Readers Insight] Why “Peak Area” is Used for Quantification in Chromatography

April 13, 2025

Peak area is the preferred metric for quantification in chromatography due to its consistency across variable peak shapes and resistance to signal distortion. Unlike peak height or volume, it offers reliable integration even with minor peak deformation or interference, making it the most robust choice in routine analysis.

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Best Practices for Mobile Phases in HPLC: Preparation, Use, and Storage

April 8, 2025

Proper preparation, handling, and storage of HPLC mobile phases are essential for achieving accurate, consistent results. This article outlines key considerations for selecting solvents, preparing mobile phases, and avoiding common pitfalls that can affect chromatographic performance and instrument longevity.

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HPLC Content Determination Method Validation: A Comprehensive Guide

March 31, 2025

This guide provides a detailed overview of HPLC method validation for content determination, highlighting key procedures, technical considerations, and common pitfalls to ensure accurate and reliable results.

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[Readers Insight] Chromatographic Separation Strategies for Multi-Component Analysis

March 28, 2025

Mass spectrometry simplifies multi-component analysis, but chromatographic separation remains essential for challenging cases. This article explores enhancements in gradient elution, column selection, temperature, flow rate, and mobile phase composition for separation efficiency and resolution.

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