In the analysis of antibody-drug conjugates, the determination of drug-to-antibody ratio is necessary. In this article, we explain why hydrophobic interaction chromatography excels in DAR determination and how to choose the column & detector and optimize the mobile phase.
This Q&A recap from our May 22 webinar covers essential questions on ADC analysis using Advanchrom HIC-Butyl columns, with expanded expert responses offering deeper insights into best practices and technical considerations.