Knowledge Base

In LC analysis, column, mobile phase, and gradient are what we pay most attention to. However, sometimes the difference in flow cells can lead to significantly different results. This article explains the details behind it.

In the second part of this article, we explain the composition of an HPLC pump and the terminologies behind, as well as practical troubleshooting and preventive maintenance methods and schedules to pump failures.

In the first part of this article, we explain common misconceptions of HPLC pumps, from the number of pump channels (and why they don't equal the number of pump heads or modules) to the differences between high- and low-pressure systems, and parallel and serial pumps.

Maintaining an HPLC system requires careful consideration of material compatibility, from 316L stainless steel pump heads to PEEK tubing. This article examines real‑world cases, compares flow‑path materials, and outlines practical cleaning and maintenance practices to safeguard analytical accuracy, prolong instrument life, minimize costly downtime, and prevent unexpected failures.

The HPLC autosampler is a key component for precise and efficient chromatography. This guide covers autosampler designs, injection methods, and essential maintenance practices to ensure accurate and reliable results.