Knowledge Base
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Advanced Chromatographic Resolution of Chiral Compounds, Part I: NSAIDs
Achieving enantiomeric purity is vital for the clinical safety and efficacy. Among the most widely prescribed analgesics, NSAIDs presents a persistent analytical challenge due to the subtle structural variations between their R- and S-enantiomers. In this article, we present the chiral separation of three significant "profen" NSAIDs (flurbiprofen, tiaprofenic acid, and carprofen) with Welch's new Blossmate chiral columns. -
Achieving Superior Isomer Separation with Welch Materials Columns
The separation of isomers is often a key challenge in HPLC due to its differences being so subtle that standard columns fail to resolve. In this article, we introduce three Welch Materials columns that perform well in isomer separation. -
Precision Characterization of Recombinant Humanized Type III Collagen: Navigating Analytical Challenges with Xtimate Bio SEC
In this article, we explore the analytical challenges of recombinant humanized type III collagen and show how Xtimate Bio SEC columns support accurate purity and aggregate profiling. The article also highlights the role of SEC in quality control and demonstrates strong chromatographic performance for collagen characterization. -
The Critical Role of Chiral Separation and Its Modern Solutions
Chirality can mean the difference between medicine and catastrophe. In this article, we summarize the necessity of enantiomeric precision and explain how our Blossmate chiral columns offer flexible, MS-compatible solutions for analytical and preparative workflows, with chromatograms comparing our and competitor's column performances. -
Advancing Metabolic Therapeutics: An Overview of Retatrutide and the Analytical Rigor of Peptide Quantification
Retatrutide is a new synthetic peptide that facilitates weight loss through a dual-action mechanism. In this article, we utilize an Ultisil LP-C18 column for the determination of Retatrutide that achieves baseline separation with high resolution records. -
![[Readers Insight] The Interplay between Column Properties and Peak Integration](//www.welch-us.com/cdn/shop/articles/integration-1080-121_535x.jpg?v=1773726738)
![[Readers Insight] The Interplay between Column Properties and Peak Integration](//www.welch-us.com/cdn/shop/articles/integration-1080-121_345x345@2x.jpg?v=1773726738)
[Readers Insight] The Interplay between Column Properties and Peak Integration
In HPLC, the relationship between physical separation hardware and mathematical data processing is often overlooked. This article explores the inconspicuous but critical connection between column properties and the fidelity of peak area calculation, examining why theoretical constancy in integration often fails to align with empirical laboratory observations. -
LP Columns: More Than Just Tolerating Strong Acids
Ultisil LP columns from Welch Materials are designed for low-pH chromatographic conditions, yet their advantages are more than simply resistance to strong acids. Read this article to learn how these columns outperform in a unique way. -
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part II)](//www.welch-us.com/cdn/shop/articles/1080-1105-2_535x.jpg?v=1773724204)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part II)](//www.welch-us.com/cdn/shop/articles/1080-1105-2_345x345@2x.jpg?v=1773724204)
[Readers Insight] HPLC Column Selection: Core to Method Development (Part II)
In this article, the author shares their answers for 8 common questions in column selection, aiming to guide users in choosing the most suitable column through practical experimentation. -
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)](//www.welch-us.com/cdn/shop/articles/columnselection2-1080-1105_535x.jpg?v=1773724222)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)](//www.welch-us.com/cdn/shop/articles/columnselection2-1080-1105_345x345@2x.jpg?v=1773724222)
[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)
A chromatographic column is the heart of separation. However, with thousands of column options on the market, it is often asked how to choose "the best one". In this article, the author explains the classifications of HPLC columns and how to select the most cmpatible column for an analyte. -
Determination of Mannitol Related Substances with Xtimate Sugar-Ca
Mannitol is a substance with important applications in pharmaceutical and food. See how Welch Xtimate Sugar-Ca columns perform well in determination of mannitol related substances in accordance with Chinese Pharmacopoeia 2025 Edition. -
Column Substitution in HPLC: More Than Just Matching Specs
When considering HPLC column replacements, cross-reference charts can only tell half the story. Read to learn how a right Welch Column goes beyond replacement—delivering optimized results tailored to your method. -
The Four Pillars of Welch Materials ODS Columns
This article explores the fundamental qualities of ODS columns and examines four representative designs from Welch Materials. It reflects on long-standing misconceptions, practical challenges, and design considerations, offering chromatographers deeper insight into one of the most widely used tools in liquid chromatography.
![[Readers Insight] The Interplay between Column Properties and Peak Integration](http://www.welch-us.com/cdn/shop/articles/integration-1080-121_535x.jpg?v=1773726738)
![[Readers Insight] The Interplay between Column Properties and Peak Integration](http://www.welch-us.com/cdn/shop/articles/integration-1080-121_345x345@2x.jpg?v=1773726738)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part II)](http://www.welch-us.com/cdn/shop/articles/1080-1105-2_535x.jpg?v=1773724204)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part II)](http://www.welch-us.com/cdn/shop/articles/1080-1105-2_345x345@2x.jpg?v=1773724204)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)](http://www.welch-us.com/cdn/shop/articles/columnselection2-1080-1105_535x.jpg?v=1773724222)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)](http://www.welch-us.com/cdn/shop/articles/columnselection2-1080-1105_345x345@2x.jpg?v=1773724222)
