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In this article, the author evaluates isocratic and gradient elution strategies in HPLC method development, providing expert guidance on selecting elution modes for pharmaceutical assays, impurity profiling, and complex separations.
![[Readers Insight] Gradient vs. Isocratic Elution: Which to Choose?](//www.welch-us.com/cdn/shop/articles/gradient-1080-105_535x.jpg?v=1767749867)
![[Readers Insight] Gradient vs. Isocratic Elution: Which to Choose?](//www.welch-us.com/cdn/shop/articles/gradient-1080-105_345x345@2x.jpg?v=1767749867)
![[Readers Insight] Gradient vs. Isocratic Elution: Which to Choose?](http://www.welch-us.com/cdn/shop/articles/gradient-1080-105_535x.jpg?v=1767749867)
![[Readers Insight] Gradient vs. Isocratic Elution: Which to Choose?](http://www.welch-us.com/cdn/shop/articles/gradient-1080-105_345x345@2x.jpg?v=1767749867)