Nicotine analysis is notoriously challenging due to its weak retention on conventional columns. This article shares a practical UHPLC-MS method using the Welch Ultisil HILIC Amphion II column, detailing the development approach, chromatographic conditions, and key advantages for reliable nicotine detection.
In this article, we explore further how matrix effects affects recovery results in mass spectrometry analyses by hindering preparation loss, and discuss when it should be corrected by using calibration curves prepared by blank matrices.
In this article, the author discusses the principles behind selecting quantifier ions in triple quadrupole mass spectrometry, with a case study to illustrate how ion choice impacts sensitivity and accuracy.
In this article, the author explains matrix curve quantification in mass spectrometry, and offers strategies to minimize matrix effects and enhance accurate quantification.
Pre-column derivatization enhances analytical sensitivity by improving chromatographic behavior, increasing ionization efficiency, and enabling optical detection for non-chromophoric compounds. This article explores how it reduces polarity, sharpens peaks, and enhances detection limits, making it a valuable tool for analyzing complex samples. By stabilizing target analytes, derivatization ensures more reliable qualitative and quantitative results in chromatography and mass spectrometry.
Discover practical strategies to tackle matrix effects in mass spectrometry, from purification to isotopic standards, ensuring accurate and reliable results.
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![[Readers Insight] Are You Choosing the Right Quantifier Ion for Mass Spectrometry?](http://www.welch-us.com/cdn/shop/articles/quantifier-ion-1080-815_19007207-6686-4fa7-9b04-bb23cb15aab5_535x.jpg?v=1760000876)
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![[Reader Insight] Optimizing Matrix Curve Quantification in Mass Spectrometry](http://www.welch-us.com/cdn/shop/articles/quantification-1080-1220_535x.webp?v=1758527555)
![[Reader Insight] Optimizing Matrix Curve Quantification in Mass Spectrometry](http://www.welch-us.com/cdn/shop/articles/quantification-1080-1220_345x345@2x.webp?v=1758527555)
![[Reader Insight] Enhancing Analytical Sensitivity with Pre-column Derivatization in Mass Spectrometry](http://www.welch-us.com/cdn/shop/articles/precolumn-1080_535x.webp?v=1752135290)
![[Reader Insight] Enhancing Analytical Sensitivity with Pre-column Derivatization in Mass Spectrometry](http://www.welch-us.com/cdn/shop/articles/precolumn-1080_345x345@2x.webp?v=1752135290)
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