Knowledge Base

[Readers Insight] How to Analyze Fragment Ions in Mass Spectrometry?

January 19, 2026

In this article, we investigate the fundamental mechanisms of ion fragmentation within mass spectrometry workflows and explore why systematically mapping fragment-ion structures is essential for deeper mass-spectrometric interpretation. It explores the relationship between electronic displacement and dissociation pathways, emphasizing structural reconstruction over simple pattern recognition.

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[Readers Insight] Why is Formic Acid Added to the Mobile Phase in Mass Spectrometry?

December 11, 2025

Formic acid is commonly seen added to the mobile phase in mass spectrometry methods, but have you wondered what specific role it plays, and can other acids substitute it? This article explains the role of formic acid in MS from the perspective of chemical reaction equilibrium thoroughly.

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A Premier Alkaloid: Nicotine and the Strategy for Developing Its Determination Method

October 9, 2025

Nicotine analysis is notoriously challenging due to its weak retention on conventional columns. This article shares a practical UHPLC-MS method using the Welch Ultisil HILIC Amphion II column, detailing the development approach, chromatographic conditions, and key advantages for reliable nicotine detection.

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[Readers Insight] Matrix Effects in Mass Spectrometry: Why They Matter

September 22, 2025

In this article, we explore further how matrix effects affects recovery results in mass spectrometry analyses by hindering preparation loss, and discuss when it should be corrected by using calibration curves prepared by blank matrices.

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[Readers Insight] Are You Choosing the Right Quantifier Ion for Mass Spectrometry?

August 20, 2025

In this article, the author discusses the principles behind selecting quantifier ions in triple quadrupole mass spectrometry, with a case study to illustrate how ion choice impacts sensitivity and accuracy.

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[Reader Insight] Optimizing Matrix Curve Quantification in Mass Spectrometry

December 20, 2024

In this article, the author explains matrix curve quantification in mass spectrometry, and offers strategies to minimize matrix effects and enhance accurate quantification.

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[Reader Insight] Enhancing Analytical Sensitivity with Pre-column Derivatization in Mass Spectrometry

November 27, 2024

Pre-column derivatization enhances analytical sensitivity by improving chromatographic behavior, increasing ionization efficiency, and enabling optical detection for non-chromophoric compounds. This article explores how it reduces polarity, sharpens peaks, and enhances detection limits, making it a valuable tool for analyzing complex samples. By stabilizing target analytes, derivatization ensures more reliable qualitative and quantitative results in chromatography and mass spectrometry.

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[Reader Insight] Matrix Effects in Mass Spectrometry Analysis: Causes and Solutions

November 21, 2024

Discover practical strategies to tackle matrix effects in mass spectrometry, from purification to isotopic standards, ensuring accurate and reliable results.

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