In this article, one of Welch’s long-term customer shares their practices and insights on pharmaceutical laboratory quality control in both HPLC and GC, along with their firsthand experience of Welch’s HPLC columns.
Peak area is the preferred metric for quantification in chromatography due to its consistency across variable peak shapes and resistance to signal distortion. Unlike peak height or volume, it offers reliable integration even with minor peak deformation or interference, making it the most robust choice in routine analysis.
Mass spectrometry simplifies multi-component analysis, but chromatographic separation remains essential for challenging cases. This article explores enhancements in gradient elution, column selection, temperature, flow rate, and mobile phase composition for separation efficiency and resolution.
![[Readers Insight] Why “Peak Area” is Used for Quantification in Chromatography](//www.welch-us.com/cdn/shop/articles/1080-peak-area_535x.webp?v=1761629420)
![[Readers Insight] Why “Peak Area” is Used for Quantification in Chromatography](//www.welch-us.com/cdn/shop/articles/1080-peak-area_345x345@2x.webp?v=1761629420)
![[Readers Insight] Chromatographic Separation Strategies for Multi-Component Analysis](//www.welch-us.com/cdn/shop/articles/1080-multi-component_535x.jpg?v=1761629958)
![[Readers Insight] Chromatographic Separation Strategies for Multi-Component Analysis](//www.welch-us.com/cdn/shop/articles/1080-multi-component_345x345@2x.jpg?v=1761629958)
![[Readers Insight] Why “Peak Area” is Used for Quantification in Chromatography](http://www.welch-us.com/cdn/shop/articles/1080-peak-area_535x.webp?v=1761629420)
![[Readers Insight] Why “Peak Area” is Used for Quantification in Chromatography](http://www.welch-us.com/cdn/shop/articles/1080-peak-area_345x345@2x.webp?v=1761629420)
![[Readers Insight] Chromatographic Separation Strategies for Multi-Component Analysis](http://www.welch-us.com/cdn/shop/articles/1080-multi-component_535x.jpg?v=1761629958)
![[Readers Insight] Chromatographic Separation Strategies for Multi-Component Analysis](http://www.welch-us.com/cdn/shop/articles/1080-multi-component_345x345@2x.jpg?v=1761629958)