Discover how derivatization technology in HPLC boosts detection and separation performance, addressing analytical challenges with innovative techniques and real-world applications.
Pre-column derivatization enhances analytical sensitivity by improving chromatographic behavior, increasing ionization efficiency, and enabling optical detection for non-chromophoric compounds. This article explores how it reduces polarity, sharpens peaks, and enhances detection limits, making it a valuable tool for analyzing complex samples. By stabilizing target analytes, derivatization ensures more reliable qualitative and quantitative results in chromatography and mass spectrometry.
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