База знаний
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Advanced Chromatographic Resolution of Chiral Compounds, Part I: NSAIDs
Achieving enantiomeric purity is vital for the clinical safety and efficacy. Among the most widely prescribed analgesics, NSAIDs presents a persistent analytical challenge due to the subtle structural variations between their R- and S-enantiomers. In this article, we present the chiral separation of three significant "profen" NSAIDs (flurbiprofen, tiaprofenic acid, and carprofen) with Welch's new Blossmate chiral columns. -
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[Readers Insight] Why Peak Fronting Occurs? Causes and Analysis
Peak fronting is a common chromatographic anomaly characterized by a gradual leading edge and a steep trailing edge, often occurring during method development. This phenomenon stems from multiple causes, thus resolving it can be much more difficult. In this article, we discuss three primary sources of peak fronting and provide technical solutions to mitigate these effects and optimize chromatographic performance. -
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![[Readers Insight] When to Use Acetone as a Mobile Phase?](//www.welch-us.com/cdn/shop/articles/acetone-1080-410_345x345@2x.jpg?v=1776059707)
[Readers Insight] When to Use Acetone as a Mobile Phase?
Acetone is a versatile solvent in chromatography, yet its application as an HPLC mobile phase remains limited to specific cases like Sudan dye analysis. This article explores the reasons behind the rare use of acetone and summarizes the key considerations when using acetone as a mobile phase. -
Achieving Superior Isomer Separation with Welch Materials Columns
The separation of isomers is often a key challenge in HPLC due to its differences being so subtle that standard columns fail to resolve. In this article, we introduce three Welch Materials columns that perform well in isomer separation. -
![[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories](//www.welch-us.com/cdn/shop/articles/pda-330-1-1080_da452ccb-f51b-4bcc-b4c5-af31d38f865c_535x.jpg?v=1775023271)
![[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories](//www.welch-us.com/cdn/shop/articles/pda-330-1-1080_da452ccb-f51b-4bcc-b4c5-af31d38f865c_345x345@2x.jpg?v=1775023271)
[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories
We all know that Mass Spectrometry is preferable for trace analysis due to its superior sensitivity. But why do PDA detectors still maintain a high utilization rate? In this article, we explore the practical advantages PDA holds compared to MS. -
The Selectivity Among Detectors: Why Mass Spectrometry Surpasses Spectroscopy?
Selectivity is a definitive factor in analytical chemistry. In this article, we discuss the differences between optical spectra and mass spectra, and explain why mass spectrometry surpasses spectroscopy in selectivity. -
Precision Characterization of Recombinant Humanized Type III Collagen: Navigating Analytical Challenges with Xtimate Bio SEC
In this article, we explore the analytical challenges of recombinant humanized type III collagen and show how Xtimate Bio SEC columns support accurate purity and aggregate profiling. The article also highlights the role of SEC in quality control and demonstrates strong chromatographic performance for collagen characterization. -
The Critical Role of Chiral Separation and Its Modern Solutions
Chirality can mean the difference between medicine and catastrophe. In this article, we summarize the necessity of enantiomeric precision and explain how our Blossmate chiral columns offer flexible, MS-compatible solutions for analytical and preparative workflows, with chromatograms comparing our and competitor's column performances. -
Advancing Metabolic Therapeutics: An Overview of Retatrutide and the Analytical Rigor of Peptide Quantification
Retatrutide is a new synthetic peptide that facilitates weight loss through a dual-action mechanism. In this article, we utilize an Ultisil LP-C18 column for the determination of Retatrutide that achieves baseline separation with high resolution records. -
![[Readers Insight] The Interplay between Column Properties and Peak Integration](//www.welch-us.com/cdn/shop/articles/integration-1080-121_535x.jpg?v=1773726738)
![[Readers Insight] The Interplay between Column Properties and Peak Integration](//www.welch-us.com/cdn/shop/articles/integration-1080-121_345x345@2x.jpg?v=1773726738)
[Readers Insight] The Interplay between Column Properties and Peak Integration
In HPLC, the relationship between physical separation hardware and mathematical data processing is often overlooked. This article explores the inconspicuous but critical connection between column properties and the fidelity of peak area calculation, examining why theoretical constancy in integration often fails to align with empirical laboratory observations. -
LP Columns: More Than Just Tolerating Strong Acids
Ultisil LP columns from Welch Materials are designed for low-pH chromatographic conditions, yet their advantages are more than simply resistance to strong acids. Read this article to learn how these columns outperform in a unique way. -
High-Resolution SEC-HPLC Aggregation Analysis of a Pembrolizumab Biosimilar Using Welch Xtimate™ SEC Columns
This application note presents a robust SEC-HPLC method for high-resolution aggregation analysis of a Pembrolizumab biosimilar using Welch Xtimate™ SEC-300 columns. The method demonstrates excellent separation of monomer, HMW aggregates, and LMW fragments, supporting routine QC and biosimilar comparability studies.
![[Readers Insight] Why Peak Fronting Occurs? Causes and Analysis](http://www.welch-us.com/cdn/shop/articles/fronting-414-1080_535x.jpg?v=1776216534)
![[Readers Insight] Why Peak Fronting Occurs? Causes and Analysis](http://www.welch-us.com/cdn/shop/articles/fronting-414-1080_345x345@2x.jpg?v=1776216534)
![[Readers Insight] When to Use Acetone as a Mobile Phase?](http://www.welch-us.com/cdn/shop/articles/acetone-1080-410_535x.jpg?v=1776059707)
![[Readers Insight] When to Use Acetone as a Mobile Phase?](http://www.welch-us.com/cdn/shop/articles/acetone-1080-410_345x345@2x.jpg?v=1776059707)
![[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories](http://www.welch-us.com/cdn/shop/articles/pda-330-1-1080_da452ccb-f51b-4bcc-b4c5-af31d38f865c_535x.jpg?v=1775023271)
![[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories](http://www.welch-us.com/cdn/shop/articles/pda-330-1-1080_da452ccb-f51b-4bcc-b4c5-af31d38f865c_345x345@2x.jpg?v=1775023271)
![[Readers Insight] The Interplay between Column Properties and Peak Integration](http://www.welch-us.com/cdn/shop/articles/integration-1080-121_535x.jpg?v=1773726738)
![[Readers Insight] The Interplay between Column Properties and Peak Integration](http://www.welch-us.com/cdn/shop/articles/integration-1080-121_345x345@2x.jpg?v=1773726738)
