База знаний
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Hydrophobic Interaction Chromatography: Your Best Teammate in the Analysis of ADCs
In the analysis of antibody-drug conjugates, the determination of drug-to-antibody ratio is necessary. In this article, we explain why hydrophobic interaction chromatography excels in DAR determination and how to choose the column & detector and optimize the mobile phase. -
Advanced Chromatographic Resolution of Chiral Compounds, Part IV: Herbicides
Herbicides are an essential tool in weed management, and chirality greatly affects their efficacy. In this article, we explore the chiral separation of two herbicides: 2-(2-chlorophenoxy)propanoic acid and dimethenamid, using Welch Blossmate chiral columns. -
Dedicate or Share? Maximize Your HPLC Column Use
In laboratory analysis, it is common to encounter scenarios where a column loses efficacy after being used in a different project, or where dozens of projects must be conducted by a small amount of columns. In this article, we explore the balance of dedicated and shared HPLC column use to ensure reliable analytical results while maximizing available resources. -
Advanced Chromatographic Resolution of Chiral Compounds, Part III: Alcohols and Diols
Alcohols and diols are among the most common functionalized intermediates in organic synthesis and pharmaceutical development, and chirality can influence their efficacy greatly. In this article, we introduce applications on the chiral separation of several typical alcohols and diol using Blossmate chiral columns. -
Mitigating Non-Specific Adsorption in Size Exclusion Chromatography: The Welch Xtimate Bio SEC Solution
Biopharmaceutical integrity relies on precise molecular size characterization. In this article, we explain how Welch Xtimate Bio SEC columns mitigate non-specific adsorption in SEC compared with competitors, and provide guides on optimizing mobile phase in different SEC assays. -
Advanced Chromatographic Resolution of Chiral Compounds, Part II: Amino Acids
Amino acids and their derivatives serve as fundamental building blocks in the synthesis of bioactive peptides and chiral drugs, and their chirality greatly affect the quality of the final product. In this article, we introduce the enantiomer separation of four different amino acid derivatives with Welch Blossmate chiral columns. -
Advanced Chromatographic Resolution of Chiral Compounds, Part I: NSAIDs
Achieving enantiomeric purity is vital for the clinical safety and efficacy. Among the most widely prescribed analgesics, NSAIDs presents a persistent analytical challenge due to the subtle structural variations between their R- and S-enantiomers. In this article, we present the chiral separation of three significant "profen" NSAIDs (flurbiprofen, tiaprofenic acid, and carprofen) with Welch's new Blossmate chiral columns. -
![[Readers Insight] Why Peak Fronting Occurs? Causes and Analysis](//www.welch-us.com/cdn/shop/articles/fronting-414-1080_535x.jpg?v=1776216534)
![[Readers Insight] Why Peak Fronting Occurs? Causes and Analysis](//www.welch-us.com/cdn/shop/articles/fronting-414-1080_345x345@2x.jpg?v=1776216534)
[Readers Insight] Why Peak Fronting Occurs? Causes and Analysis
Peak fronting is a common chromatographic anomaly characterized by a gradual leading edge and a steep trailing edge, often occurring during method development. This phenomenon stems from multiple causes, thus resolving it can be much more difficult. In this article, we discuss three primary sources of peak fronting and provide technical solutions to mitigate these effects and optimize chromatographic performance. -
![[Readers Insight] When to Use Acetone as a Mobile Phase?](//www.welch-us.com/cdn/shop/articles/acetone-1080-410_535x.jpg?v=1776059707)
![[Readers Insight] When to Use Acetone as a Mobile Phase?](//www.welch-us.com/cdn/shop/articles/acetone-1080-410_345x345@2x.jpg?v=1776059707)
[Readers Insight] When to Use Acetone as a Mobile Phase?
Acetone is a versatile solvent in chromatography, yet its application as an HPLC mobile phase remains limited to specific cases like Sudan dye analysis. This article explores the reasons behind the rare use of acetone and summarizes the key considerations when using acetone as a mobile phase. -
Achieving Superior Isomer Separation with Welch Materials Columns
The separation of isomers is often a key challenge in HPLC due to its differences being so subtle that standard columns fail to resolve. In this article, we introduce three Welch Materials columns that perform well in isomer separation. -
![[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories](//www.welch-us.com/cdn/shop/articles/pda-330-1-1080_da452ccb-f51b-4bcc-b4c5-af31d38f865c_535x.jpg?v=1775023271)
![[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories](//www.welch-us.com/cdn/shop/articles/pda-330-1-1080_da452ccb-f51b-4bcc-b4c5-af31d38f865c_345x345@2x.jpg?v=1775023271)
[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories
We all know that Mass Spectrometry is preferable for trace analysis due to its superior sensitivity. But why do PDA detectors still maintain a high utilization rate? In this article, we explore the practical advantages PDA holds compared to MS. -
The Selectivity Among Detectors: Why Mass Spectrometry Surpasses Spectroscopy?
Selectivity is a definitive factor in analytical chemistry. In this article, we discuss the differences between optical spectra and mass spectra, and explain why mass spectrometry surpasses spectroscopy in selectivity.
![[Readers Insight] Why Peak Fronting Occurs? Causes and Analysis](http://www.welch-us.com/cdn/shop/articles/fronting-414-1080_535x.jpg?v=1776216534)
![[Readers Insight] Why Peak Fronting Occurs? Causes and Analysis](http://www.welch-us.com/cdn/shop/articles/fronting-414-1080_345x345@2x.jpg?v=1776216534)
![[Readers Insight] When to Use Acetone as a Mobile Phase?](http://www.welch-us.com/cdn/shop/articles/acetone-1080-410_535x.jpg?v=1776059707)
![[Readers Insight] When to Use Acetone as a Mobile Phase?](http://www.welch-us.com/cdn/shop/articles/acetone-1080-410_345x345@2x.jpg?v=1776059707)
![[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories](http://www.welch-us.com/cdn/shop/articles/pda-330-1-1080_da452ccb-f51b-4bcc-b4c5-af31d38f865c_535x.jpg?v=1775023271)
![[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories](http://www.welch-us.com/cdn/shop/articles/pda-330-1-1080_da452ccb-f51b-4bcc-b4c5-af31d38f865c_345x345@2x.jpg?v=1775023271)
