База знаний
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Advanced Chromatographic Resolution of Chiral Compounds, Part I: NSAIDs
Achieving enantiomeric purity is vital for the clinical safety and efficacy. Among the most widely prescribed analgesics, NSAIDs presents a persistent analytical challenge due to the subtle structural variations between their R- and S-enantiomers. In this article, we present the chiral separation of three significant "profen" NSAIDs (flurbiprofen, tiaprofenic acid, and carprofen) with Welch's new Blossmate chiral columns. -
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[Readers Insight] Why Peak Fronting Occurs? Causes and Analysis
Peak fronting is a common chromatographic anomaly characterized by a gradual leading edge and a steep trailing edge, often occurring during method development. This phenomenon stems from multiple causes, thus resolving it can be much more difficult. In this article, we discuss three primary sources of peak fronting and provide technical solutions to mitigate these effects and optimize chromatographic performance. -
![[Readers Insight] When to Use Acetone as a Mobile Phase?](//www.welch-us.com/cdn/shop/articles/acetone-1080-410_535x.jpg?v=1776059707)
![[Readers Insight] When to Use Acetone as a Mobile Phase?](//www.welch-us.com/cdn/shop/articles/acetone-1080-410_345x345@2x.jpg?v=1776059707)
[Readers Insight] When to Use Acetone as a Mobile Phase?
Acetone is a versatile solvent in chromatography, yet its application as an HPLC mobile phase remains limited to specific cases like Sudan dye analysis. This article explores the reasons behind the rare use of acetone and summarizes the key considerations when using acetone as a mobile phase. -
Achieving Superior Isomer Separation with Welch Materials Columns
The separation of isomers is often a key challenge in HPLC due to its differences being so subtle that standard columns fail to resolve. In this article, we introduce three Welch Materials columns that perform well in isomer separation. -
![[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories](//www.welch-us.com/cdn/shop/articles/pda-330-1-1080_da452ccb-f51b-4bcc-b4c5-af31d38f865c_535x.jpg?v=1775023271)
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[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories
We all know that Mass Spectrometry is preferable for trace analysis due to its superior sensitivity. But why do PDA detectors still maintain a high utilization rate? In this article, we explore the practical advantages PDA holds compared to MS. -
The Selectivity Among Detectors: Why Mass Spectrometry Surpasses Spectroscopy?
Selectivity is a definitive factor in analytical chemistry. In this article, we discuss the differences between optical spectra and mass spectra, and explain why mass spectrometry surpasses spectroscopy in selectivity. -
Beyond the Enclosure: Redefining Explosion-Proof Preparative HPLC Systems
Explosion-proof preparative HPLC is no longer just about a flameproof cabinet. In biopharmaceutical production, true safety means integrating explosion protection, hygienic design, and GMP-ready compliance across the entire system—from fluid path to software. Read this article to see how Welch Materials' WelPacker DAC provides a trustworthy, high-efficiency solution for the complex challenges of purification in hazardous environments. -
Precision Characterization of Recombinant Humanized Type III Collagen: Navigating Analytical Challenges with Xtimate Bio SEC
In this article, we explore the analytical challenges of recombinant humanized type III collagen and show how Xtimate Bio SEC columns support accurate purity and aggregate profiling. The article also highlights the role of SEC in quality control and demonstrates strong chromatographic performance for collagen characterization. -
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![[Readers Insight] Is "Not Detected" Truly "Not Detected"? (Not An Article about LOD)](//www.welch-us.com/cdn/shop/articles/1080-3182_2579cff7-26a7-4813-bab4-560f24b5e3be_345x345@2x.jpg?v=1773819396)
[Readers Insight] Is "Not Detected" Truly "Not Detected"? (Not An Article about LOD)
When you encounter "Not Detected" in analytical chemistry, does it truly mean the substance is absent? In practice, unscrupulous manufacturers may try to evade detection of prohibited additives by modifying molecular structures to something out of the detection scope. In this article, the author introduces a method using TOF-IDA/MS to effectively expose such modification without the need of reference standards. -
The Critical Role of Chiral Separation and Its Modern Solutions
Chirality can mean the difference between medicine and catastrophe. In this article, we summarize the necessity of enantiomeric precision and explain how our Blossmate chiral columns offer flexible, MS-compatible solutions for analytical and preparative workflows, with chromatograms comparing our and competitor's column performances. -
Advancing Metabolic Therapeutics: An Overview of Retatrutide and the Analytical Rigor of Peptide Quantification
Retatrutide is a new synthetic peptide that facilitates weight loss through a dual-action mechanism. In this article, we utilize an Ultisil LP-C18 column for the determination of Retatrutide that achieves baseline separation with high resolution records. -
![[Readers Insight] How to Analyze Fragment Ions in Mass Spectrometry?](//www.welch-us.com/cdn/shop/articles/frag-ion-120-1080_535x.jpg?v=1773726852)
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[Readers Insight] How to Analyze Fragment Ions in Mass Spectrometry?
In this article, we investigate the fundamental mechanisms of ion fragmentation within mass spectrometry workflows and explore why systematically mapping fragment-ion structures is essential for deeper mass-spectrometric interpretation. It explores the relationship between electronic displacement and dissociation pathways, emphasizing structural reconstruction over simple pattern recognition.
![[Readers Insight] Why Peak Fronting Occurs? Causes and Analysis](http://www.welch-us.com/cdn/shop/articles/fronting-414-1080_535x.jpg?v=1776216534)
![[Readers Insight] Why Peak Fronting Occurs? Causes and Analysis](http://www.welch-us.com/cdn/shop/articles/fronting-414-1080_345x345@2x.jpg?v=1776216534)
![[Readers Insight] When to Use Acetone as a Mobile Phase?](http://www.welch-us.com/cdn/shop/articles/acetone-1080-410_535x.jpg?v=1776059707)
![[Readers Insight] When to Use Acetone as a Mobile Phase?](http://www.welch-us.com/cdn/shop/articles/acetone-1080-410_345x345@2x.jpg?v=1776059707)
![[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories](http://www.welch-us.com/cdn/shop/articles/pda-330-1-1080_da452ccb-f51b-4bcc-b4c5-af31d38f865c_535x.jpg?v=1775023271)
![[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories](http://www.welch-us.com/cdn/shop/articles/pda-330-1-1080_da452ccb-f51b-4bcc-b4c5-af31d38f865c_345x345@2x.jpg?v=1775023271)
![[Readers Insight] Is](http://www.welch-us.com/cdn/shop/articles/1080-3182_2579cff7-26a7-4813-bab4-560f24b5e3be_535x.jpg?v=1773819396)
![[Readers Insight] Is "Not Detected" Truly "Not Detected"? (Not An Article about LOD)](http://www.welch-us.com/cdn/shop/articles/1080-3182_2579cff7-26a7-4813-bab4-560f24b5e3be_345x345@2x.jpg?v=1773819396)
![[Readers Insight] How to Analyze Fragment Ions in Mass Spectrometry?](http://www.welch-us.com/cdn/shop/articles/frag-ion-120-1080_535x.jpg?v=1773726852)
![[Readers Insight] How to Analyze Fragment Ions in Mass Spectrometry?](http://www.welch-us.com/cdn/shop/articles/frag-ion-120-1080_345x345@2x.jpg?v=1773726852)