База знаний
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On World Chocolate Day: Analyzing Disodium EDTA in Chocolate by HPLC
Chocolate is one of the most consumed sweet on Earth, and its quality and safety is a major consideration. However, chocolate's component greatly interfere with chromatographic analysis. In this article, we present an HPLC method with trichloromethane pretreatment to analyze Disodium EDTA in chocolate. -
Welch Solution for Biological Sample Analysis
In this article, we present solutions for the chromatographic analysis of biological samples from Welch Materials, covering SEC, RPLC, HIC, and ion exchange chromatography, with multiple applications of peptides, nucleic acids, antibodies, and proteins. -
Advanced Chromatographic Resolution of Chiral Compounds, Part VI: Intermediates
While synthetic intermediates are not themselves in the final API, their stereochemical purity affects product quality greatly. In this article, we present application notes of five typical synthetic intermediates, witnessing how Blossmate chiral columns successfully separate them from their enantiomers. -
Advanced Chromatographic Resolution of Chiral Compounds, Part V: APIs
More than half of APIs contain chiral centers, which may hinder drug activity if not separated. In this article, we use Blossmate chiral columns to separate five distinct APIs with their enantiomers, showcasing the columns' capability in such applications. -
A Simple and Efficient Solution for HPLC Sensitivity: The WelView Photo Chemical Reactor
In chromatographic analysis, achieving high sensitivity and selectivity for trace-level analytes within complex matrices is a foundational challenge. In this article, we introduce WelView Photo Chemical Reactor and explore how it enhances HPLC sensitivity with post-column derivatization through two examples. -
A Comparison of Welch Ultisil Columns on the Isomeric Separation of Anthraquinones
Isomer separation is one of the most challenging tasks in chromatography. In this article, we evaluate the chromatographic performance of three Welch Materials’ Ultisil stationary phases—PFP, Alk-C18, and XS-C18—on the separation of isomeric derivatives and analogs of antharaquinone. -
Advanced Chromatographic Resolution of Chiral Compounds, Part IV: Herbicides
Herbicides are an essential tool in weed management, and chirality greatly affects their efficacy. In this article, we explore the chiral separation of two herbicides: 2-(2-chlorophenoxy)propanoic acid and dimethenamid, using Welch Blossmate chiral columns. -
Advanced Chromatographic Resolution of Chiral Compounds, Part III: Alcohols and Diols
Alcohols and diols are among the most common functionalized intermediates in organic synthesis and pharmaceutical development, and chirality can influence their efficacy greatly. In this article, we introduce applications on the chiral separation of several typical alcohols and diol using Blossmate chiral columns. -
Mitigating Non-Specific Adsorption in Size Exclusion Chromatography: The Welch Xtimate Bio SEC Solution
Biopharmaceutical integrity relies on precise molecular size characterization. In this article, we explain how Welch Xtimate Bio SEC columns mitigate non-specific adsorption in SEC compared with competitors, and provide guides on optimizing mobile phase in different SEC assays. -
Advanced Chromatographic Resolution of Chiral Compounds, Part II: Amino Acids
Amino acids and their derivatives serve as fundamental building blocks in the synthesis of bioactive peptides and chiral drugs, and their chirality greatly affect the quality of the final product. In this article, we introduce the enantiomer separation of four different amino acid derivatives with Welch Blossmate chiral columns. -
Advanced Chromatographic Resolution of Chiral Compounds, Part I: NSAIDs
Achieving enantiomeric purity is vital for the clinical safety and efficacy. Among the most widely prescribed analgesics, NSAIDs presents a persistent analytical challenge due to the subtle structural variations between their R- and S-enantiomers. In this article, we present the chiral separation of three significant "profen" NSAIDs (flurbiprofen, tiaprofenic acid, and carprofen) with Welch's new Blossmate chiral columns. -
Achieving Superior Isomer Separation with Welch Materials Columns
The separation of isomers is often a key challenge in HPLC due to its differences being so subtle that standard columns fail to resolve. In this article, we introduce three Welch Materials columns that perform well in isomer separation.
