База знаний
-
![[Readers Insight] When to Use Acetone as a Mobile Phase?](//www.welch-us.com/cdn/shop/articles/acetone-1080-410_535x.jpg?v=1776059707)
![[Readers Insight] When to Use Acetone as a Mobile Phase?](//www.welch-us.com/cdn/shop/articles/acetone-1080-410_345x345@2x.jpg?v=1776059707)
[Readers Insight] When to Use Acetone as a Mobile Phase?
Acetone is a versatile solvent in chromatography, yet its application as an HPLC mobile phase remains limited to specific cases like Sudan dye analysis. This article explores the reasons behind the rare use of acetone and summarizes the key considerations when using acetone as a mobile phase. -
Achieving Superior Isomer Separation with Welch Materials Columns
The separation of isomers is often a key challenge in HPLC due to its differences being so subtle that standard columns fail to resolve. In this article, we introduce three Welch Materials columns that perform well in isomer separation. -
![[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories](//www.welch-us.com/cdn/shop/articles/pda-330-1-1080_da452ccb-f51b-4bcc-b4c5-af31d38f865c_535x.jpg?v=1775023271)
![[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories](//www.welch-us.com/cdn/shop/articles/pda-330-1-1080_da452ccb-f51b-4bcc-b4c5-af31d38f865c_345x345@2x.jpg?v=1775023271)
[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories
We all know that Mass Spectrometry is preferable for trace analysis due to its superior sensitivity. But why do PDA detectors still maintain a high utilization rate? In this article, we explore the practical advantages PDA holds compared to MS. -
The Selectivity Among Detectors: Why Mass Spectrometry Surpasses Spectroscopy?
Selectivity is a definitive factor in analytical chemistry. In this article, we discuss the differences between optical spectra and mass spectra, and explain why mass spectrometry surpasses spectroscopy in selectivity. -
Beyond the Enclosure: Redefining Explosion-Proof Preparative HPLC Systems
Explosion-proof preparative HPLC is no longer just about a flameproof cabinet. In biopharmaceutical production, true safety means integrating explosion protection, hygienic design, and GMP-ready compliance across the entire system—from fluid path to software. Read this article to see how Welch Materials' WelPacker DAC provides a trustworthy, high-efficiency solution for the complex challenges of purification in hazardous environments. -
Precision Characterization of Recombinant Humanized Type III Collagen: Navigating Analytical Challenges with Xtimate Bio SEC
In this article, we explore the analytical challenges of recombinant humanized type III collagen and show how Xtimate Bio SEC columns support accurate purity and aggregate profiling. The article also highlights the role of SEC in quality control and demonstrates strong chromatographic performance for collagen characterization. -
![[Readers Insight] Is](//www.welch-us.com/cdn/shop/articles/1080-3182_2579cff7-26a7-4813-bab4-560f24b5e3be_535x.jpg?v=1773819396)
![[Readers Insight] Is "Not Detected" Truly "Not Detected"? (Not An Article about LOD)](//www.welch-us.com/cdn/shop/articles/1080-3182_2579cff7-26a7-4813-bab4-560f24b5e3be_345x345@2x.jpg?v=1773819396)
[Readers Insight] Is "Not Detected" Truly "Not Detected"? (Not An Article about LOD)
When you encounter "Not Detected" in analytical chemistry, does it truly mean the substance is absent? In practice, unscrupulous manufacturers may try to evade detection of prohibited additives by modifying molecular structures to something out of the detection scope. In this article, the author introduces a method using TOF-IDA/MS to effectively expose such modification without the need of reference standards. -
The Critical Role of Chiral Separation and Its Modern Solutions
Chirality can mean the difference between medicine and catastrophe. In this article, we summarize the necessity of enantiomeric precision and explain how our Blossmate chiral columns offer flexible, MS-compatible solutions for analytical and preparative workflows, with chromatograms comparing our and competitor's column performances. -
Advancing Metabolic Therapeutics: An Overview of Retatrutide and the Analytical Rigor of Peptide Quantification
Retatrutide is a new synthetic peptide that facilitates weight loss through a dual-action mechanism. In this article, we utilize an Ultisil LP-C18 column for the determination of Retatrutide that achieves baseline separation with high resolution records. -
![[Readers Insight] How to Analyze Fragment Ions in Mass Spectrometry?](//www.welch-us.com/cdn/shop/articles/frag-ion-120-1080_535x.jpg?v=1773726852)
![[Readers Insight] How to Analyze Fragment Ions in Mass Spectrometry?](//www.welch-us.com/cdn/shop/articles/frag-ion-120-1080_345x345@2x.jpg?v=1773726852)
[Readers Insight] How to Analyze Fragment Ions in Mass Spectrometry?
In this article, we investigate the fundamental mechanisms of ion fragmentation within mass spectrometry workflows and explore why systematically mapping fragment-ion structures is essential for deeper mass-spectrometric interpretation. It explores the relationship between electronic displacement and dissociation pathways, emphasizing structural reconstruction over simple pattern recognition. -
![[Readers Insight] Asking the Questions Right: A Guide to Troubleshooting Consultations in HPLC Method Development](//www.welch-us.com/cdn/shop/articles/asking-115-1080_535x.jpg?v=1773726811)
![[Readers Insight] Asking the Questions Right: A Guide to Troubleshooting Consultations in HPLC Method Development](//www.welch-us.com/cdn/shop/articles/asking-115-1080_345x345@2x.jpg?v=1773726811)
[Readers Insight] Asking the Questions Right: A Guide to Troubleshooting Consultations in HPLC Method Development
Mastering HPLC method development requires more than technical skill; it demands effective communication. This guide explores how junior scientists can overcome "question anxiety" by utilizing precise data packages and structured inquiries. Learn to transform troubleshooting consultations into professional growth opportunities, ensuring faster resolutions and building lasting laboratory expertise. -
![[Readers Insight] The Interplay between Column Properties and Peak Integration](//www.welch-us.com/cdn/shop/articles/integration-1080-121_535x.jpg?v=1773726738)
![[Readers Insight] The Interplay between Column Properties and Peak Integration](//www.welch-us.com/cdn/shop/articles/integration-1080-121_345x345@2x.jpg?v=1773726738)
[Readers Insight] The Interplay between Column Properties and Peak Integration
In HPLC, the relationship between physical separation hardware and mathematical data processing is often overlooked. This article explores the inconspicuous but critical connection between column properties and the fidelity of peak area calculation, examining why theoretical constancy in integration often fails to align with empirical laboratory observations.
![[Readers Insight] When to Use Acetone as a Mobile Phase?](http://www.welch-us.com/cdn/shop/articles/acetone-1080-410_535x.jpg?v=1776059707)
![[Readers Insight] When to Use Acetone as a Mobile Phase?](http://www.welch-us.com/cdn/shop/articles/acetone-1080-410_345x345@2x.jpg?v=1776059707)
![[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories](http://www.welch-us.com/cdn/shop/articles/pda-330-1-1080_da452ccb-f51b-4bcc-b4c5-af31d38f865c_535x.jpg?v=1775023271)
![[Readers Insight] Why Does the PDA Detector Remain a Cornerstone of Analytical Laboratories](http://www.welch-us.com/cdn/shop/articles/pda-330-1-1080_da452ccb-f51b-4bcc-b4c5-af31d38f865c_345x345@2x.jpg?v=1775023271)
![[Readers Insight] Is](http://www.welch-us.com/cdn/shop/articles/1080-3182_2579cff7-26a7-4813-bab4-560f24b5e3be_535x.jpg?v=1773819396)
![[Readers Insight] Is "Not Detected" Truly "Not Detected"? (Not An Article about LOD)](http://www.welch-us.com/cdn/shop/articles/1080-3182_2579cff7-26a7-4813-bab4-560f24b5e3be_345x345@2x.jpg?v=1773819396)
![[Readers Insight] How to Analyze Fragment Ions in Mass Spectrometry?](http://www.welch-us.com/cdn/shop/articles/frag-ion-120-1080_535x.jpg?v=1773726852)
![[Readers Insight] How to Analyze Fragment Ions in Mass Spectrometry?](http://www.welch-us.com/cdn/shop/articles/frag-ion-120-1080_345x345@2x.jpg?v=1773726852)
![[Readers Insight] Asking the Questions Right: A Guide to Troubleshooting Consultations in HPLC Method Development](http://www.welch-us.com/cdn/shop/articles/asking-115-1080_535x.jpg?v=1773726811)
![[Readers Insight] Asking the Questions Right: A Guide to Troubleshooting Consultations in HPLC Method Development](http://www.welch-us.com/cdn/shop/articles/asking-115-1080_345x345@2x.jpg?v=1773726811)
![[Readers Insight] The Interplay between Column Properties and Peak Integration](http://www.welch-us.com/cdn/shop/articles/integration-1080-121_535x.jpg?v=1773726738)
![[Readers Insight] The Interplay between Column Properties and Peak Integration](http://www.welch-us.com/cdn/shop/articles/integration-1080-121_345x345@2x.jpg?v=1773726738)