Common problems occurred in the use of HPLC columns HPLC column is the "HEART" of the chromatographic system. Due to the wide variety of samples and the complex matrix of...
C18 is retentive to non-polar compounds as the non-polarity of C18 group, but for polar compounds, sometimes the mobile phase with high water ratio should be used to make the...
It’s usually very difficult to identify the twins, but mothers can easily identify their twins according to habits and characteristics. Isomer In Chemical world, there also exist some compounds with...
Isomers are compounds with similar structures and their chemical equilibrium constant(K) is almost the same. It’s very difficult to operate chromatography separation on isomers, especially the separation of meta and...
Today we will bring “Solutions to solve poor reproducibility between batch to batch”. Q How to ensure good reproducibility when a new validated HPLC method is transferred to Q.C. lab?...
