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His-tagged protein purification with Ni column, so simple!
Introduction In 1975, Porath et al. proposed a new purification method - immobilized metal chelate chromatography. This method uses coordination-chelation interaction of metal ions (Ni2+, Cu2+, etc.) with residues... -
Sharing a Purification Process Scheme for High-Purity RNase A
Ribonuclease A Ribonuclease A (RNase A), abbreviated as RNase A, is a single-chain polypeptide containing four disulfide bonds, with a molecular weight of approximately 13.7 kDa (amino acid sequence). RNase...