Introduction In 1975, Porath et al. proposed a new purification method - immobilized metal chelate chromatography. This method uses coordination-chelation interaction of metal ions (Ni2+, Cu2+, etc.) with residues...
Ribonuclease A Ribonuclease A (RNase A), abbreviated as RNase A, is a single-chain polypeptide containing four disulfide bonds, with a molecular weight of approximately 13.7 kDa (amino acid sequence). RNase...