News
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Flushing and Regeneration of Column Based on Polymer
Introduction of Column Based on Polymer Polymer matrix columns (Sugar-H, Sugar-Ca) may be contaminated, resulting in decreased column efficiency. Thus, flushing and regeneration are required. However, when flushing these columns... -
Poor Peak Shape, and How to Improve It
When the injection volume of test substance within the range of the security, changing injection volume will affect the peak height and peak area accordingly. But the influence of retention... -
How to Select a Suitable Column?
When you look up the pharmacopoeia method, you may find that it only stipulates the bonding phase of column and the information of other columns is not available. Therefore, you... -
Methods for Prolonging the Column Lifetime
Your column may suffer from short working life, which not only causes rising costs, but also impacts the experimental results. Today, we invite Welch engineers to answer questions about how... -
Gel Purification Chromatography
Gel Purification Chromatography Gel chromatography is a separation and analysis technology developed in the early 1960s. According to whether the separated objects are water-soluble substances, gel chromatography can be divided... -
Flushing and Regeneration of Reversed-Phase Column
Do you know the maintenance methods of Welch columns? Some people may use the wrong way to flush columns unconsciously, so it is recommended to learn some practical methods for... -
How to Maintain Our Non-Reversed Phase Columns?
Pollution, loss and other problems will inevitably appear during the use of HPLC columns, thus it is necessary to flush column for maintenance at that time. From the point of... -
How Can Polar Compounds be Better Retained?
Reversed-phase columns usually take silica gel, organic/ inorganic hybrid silica gel or polymer as the matrix, while C18 as the bonded phase. Based on the hydrophobic interaction between analytes and... -
Distinction of Related Concepts About Liquid Chromatography
High performance liquid chromatography (HPLC) is a fundamental analytical discipline with many similar concepts. Therefore, it’s necessary for us to learn how to distinguish some easily-confused concepts of liquid chromatography.... -
The principle of 100% water compatible column
There are many kinds of C18 columns in the market, some are 100% water compatible, some are not. Why can they be used at 100% water? How to distinguish? What... -
How to distinguish Guard column and Pre-column Inline Filter?
Guard column and Pre-column inline filter are commonly used in LC experiments. But a lot of users are confused about the two products as they look similar. How to distinguish?... -
Why is there always a "ghost" in your chromatogram?
What’s Ghost Peaks? In the chromatographic separation, there are some peaks which appears erratically like ghosts during gradient elution or long-period operation, we call them Ghost Peaks. The harm of...