Knowledge Base

In recent high-impact publications on untargeted metabolomics, lipidomics, oligonucleotide therapeutics, and pesticide residue analysis, ammonium fluoride (NH4F) is a frequently encountered mobile phase additive. In this article, we will explore why it improves sensitivity in negative-mode LC-MS, and how it should be used to avoid compromising performance or damaging LC systems.

High-purity phosphoramidite monomers are essential for reliable oligonucleotide manufacturing. In this article, we present applications evaluating Welch Materials Advanchrom C18 and Xtimate C18 for phosphoramidite purity analysis, demonstrating excellent resolution, high column efficiency, and chromatographic purities above 98%.

In chromatographic practice, we often discard the data from the first injection because it is unreliable. But why? In this article, we discuss the reasons that affects the results of the first injection, and why running a blank injection is more rigorous.

In this article, we review and analyze one of the tasks on the practical stage of the 4th Welch Materials Cup, Separation of Multiple Polar Nucleobases. We thoroughly analyze the key challenges during the method development where participants may easily lose points, briefly explain how participants are scored in the competition, and the logic in HPLC method development.

In porous chromatographic packing materials, more than 95% of interactions between the stationary phase and target analytes occur within the pores, which makes pore size one of the most important parameters of a packing material. In this article, we discuss the relationship between column pore size and target molecular weight, and share a real-world application case where a 300Å column outperform a 120Å column in the analysis of a large molecular weight analyte.

The 2026 Welch Materials Cup Chromatography Competition, held in China from July to August 2026, featured a number of questions designed to assess more than simple knowledge recall. Many of the questions focused on the ability to connect chromatographic theory with practical applications and to apply fundamental principles when faced with complex analytical conditions. In this article, we have selected 11 representative questions that showed the highest error rates and translated both the questions and their detailed analyses into English.

Neotame is a food additive widely used in various food products. Due to its potential adverse effects, its use must comply with the maximum usage limits and permitted categories specified by relevant regulations. In this article, we introduce our solution using SPE-HPLC and SPE-LC-MS/MS, based on the Chinese national food safety standard GB 5009.247-2025.

Chirality, the handedness in chemistry, can mean a significant difference between life-saver and toxicity. In this article, we not only explore how chiral HPLC separates enantiomers with its unique three-point interaction mechanism, but also briefly introduce five brand new chiral stationary phases in Blossmate Chiral series, greatly expanding Welch Materials' chiral chromatography solutions.

In analytical chemistry, spiked recovery rate is a fundamental parameter used to evaluate the accuracy of an analytical method. In this article, we explore the three categories of spiked recovery experiments, the calculation of spiked recovery rate and required spiking amount, and the practical experimental guidelines for a valid experiment.

The sugar column used in carbohydrate and sugar analysis has different operational precautions than other commonly used chromatographic columns. Many users, even after reading the product manual, still have difficulties on how to properly activate, use, and store them. In this article, we break down the details of using sugar columns

In introductory liquid chromatography training, almost every beginner has heard the following statement: “In reversed-phase chromatography, more polar compounds elute first, while less polar compounds elute later.” But chromatography is not that simple. In this article, we discuss factors beyond polarity that determines elution order.

Cereulide is an emetic toxin found in grains and dairy products that requires prevention and control. In this article, we present a method that quantifies cereulide in infant formula and rice cereal, using solid-phase extraction, UHPLC, and mass spectrometry.