Knowledge Base

In this article, we explore a simple yet effective trick in the elution program for isomer separation, with two examples to show how this trick works.

A real-world case of poor reproducibility in LC analysis, with a general conclusion of common issues that cause RSD to be high.

Explore the mechanism of ion-pairing reagents, and common issues and solutions when using ion-pairing chromatography.

Introduce the principle of amino columns (nh2 columns) and the guidelines for optimizing amino column performance in reversed-phase and normal-phase mode.

In this article, we share an application of an analytical method for 18 amino acids. Derivatization is employed in this method, and Welch Ultisil Amino Acid Column is used for analysis.

In this article, we explore how particle size affects resolution in liquid chromatography through two real-world applications, and analyze why these cases show different results from common belief.

Learn how to identify and address baseline noise and drift in chromatography, understand their causes, and apply effective solutions for UV detectors.

This article explores key derivatization methods like methylation, amidation, and esterification, highlighting their principles, applications, and optimization strategies.

This article introduces dead volume in HPLC: its definition, calculation methods, and tips to reduce it for better peak shapes and chromatographic efficiency.

Learn about chaotropic agents in RPLC, their mechanisms, and benefits over ion-pair reagents, including improved retention, reproducibility, and column integrity.

Discover how derivatization technology in HPLC boosts detection and separation performance, addressing analytical challenges with innovative techniques and real-world applications.

Welch Xtimate Sugar Columns offer precise carbohydrate separation via ion coordination exchange, with easy regeneration and protection from metal ion interference.