Knowledge Base
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![[Readers Insight] HPLC Column Selection: Core to Method Development (Part II)](//www.welch-us.com/cdn/shop/articles/1080-1105-2_535x.jpg?v=1762395398)
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[Readers Insight] HPLC Column Selection: Core to Method Development (Part II)
In this article, the author shares their answers for 8 common questions in column selection, aiming to guide users in choosing the most suitable column through practical experimentation. -
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)](//www.welch-us.com/cdn/shop/articles/columnselection2-1080-1105_535x.jpg?v=1762395940)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)](//www.welch-us.com/cdn/shop/articles/columnselection2-1080-1105_345x345@2x.jpg?v=1762395940)
[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)
A chromatographic column is the heart of separation. However, with thousands of column options on the market, it is often asked how to choose "the best one". In this article, the author explains the classifications of HPLC columns and how to select the most cmpatible column for an analyte. -
Determination of Mannitol Related Substances with Xtimate Sugar-Ca
Mannitol is a substance with important applications in pharmaceutical and food. See how Welch Xtimate Sugar-Ca columns perform well in determination of mannitol related substances in accordance with Chinese Pharmacopoeia 2025 Edition. -
Column Substitution in HPLC: More Than Just Matching Specs
When considering HPLC column replacements, cross-reference charts can only tell half the story. Read to learn how a right Welch Column goes beyond replacement—delivering optimized results tailored to your method. -
The Four Pillars of Welch Materials ODS Columns
This article explores the fundamental qualities of ODS columns and examines four representative designs from Welch Materials. It reflects on long-standing misconceptions, practical challenges, and design considerations, offering chromatographers deeper insight into one of the most widely used tools in liquid chromatography. -
Preventing Hydrophobic Collapse: Demystifying AQ Columns
Struggling with weak retention of polar compounds in RP‑HPLC? Discover how Ultisil® AQ‑C18 delivers reliable performance under 100% aqueous conditions in this article. Enhance your analyses with stable retention and extended column life. -
Sugar Columns in Series: Empowering Superior Separation Performance in Sugar Analysis
This article explores how using Xtimate® Sugar-H and Sugar-Ca columns in series can significantly enhance resolution in sugar analysis. With real application cases in fermentation broth and pharmaceutical solutions, it demonstrates improved separation performance while maintaining safe pressure levels—offering a practical solution for challenging HPLC sugar separations. -
Advanchrom HIC-Butyl FAQ: Linker Stability, Sample Handling, Column Treatment…
This Q&A recap from our May 22 webinar covers essential questions on ADC analysis using Advanchrom HIC-Butyl columns, with expanded expert responses offering deeper insights into best practices and technical considerations. -
Welch Columns in the Spotlight: Shining Stars of the 2025 Edition of the Chinese Pharmacopoeia
Discover Welch HPLC columns featured in the 2025 Chinese Pharmacopoeia, with applications and specs for pharmaceutical analysis. -
Amino (NH2) Column: How to Keep High Performance?
Introduce the principle of amino columns (nh2 columns) and the guidelines for optimizing amino column performance in reversed-phase and normal-phase mode. -
Does Smaller Column Particle Size Always Lead to Better Resolution in Liquid Chromatography?
In this article, we explore how particle size affects resolution in liquid chromatography through two real-world applications, and analyze why these cases show different results from common belief. -
Unlocking the Potential of Derivatization Technology in HPLC Analysis
Discover how derivatization technology in HPLC boosts detection and separation performance, addressing analytical challenges with innovative techniques and real-world applications.
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part II)](http://www.welch-us.com/cdn/shop/articles/1080-1105-2_535x.jpg?v=1762395398)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part II)](http://www.welch-us.com/cdn/shop/articles/1080-1105-2_345x345@2x.jpg?v=1762395398)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)](http://www.welch-us.com/cdn/shop/articles/columnselection2-1080-1105_535x.jpg?v=1762395940)
![[Readers Insight] HPLC Column Selection: Core to Method Development (Part I)](http://www.welch-us.com/cdn/shop/articles/columnselection2-1080-1105_345x345@2x.jpg?v=1762395940)
