Knowledge Base

Achieving enantiomeric purity is vital for the clinical safety and efficacy. Among the most widely prescribed analgesics, NSAIDs presents a persistent analytical challenge due to the subtle structural variations between their R- and S-enantiomers. In this article, we present the chiral separation of three significant "profen" NSAIDs (flurbiprofen, tiaprofenic acid, and carprofen) with Welch's new Blossmate chiral columns.

The separation of isomers is often a key challenge in HPLC due to its differences being so subtle that standard columns fail to resolve. In this article, we introduce three Welch Materials columns that perform well in isomer separation.

In this article, we explore the analytical challenges of recombinant humanized type III collagen and show how Xtimate Bio SEC columns support accurate purity and aggregate profiling. The article also highlights the role of SEC in quality control and demonstrates strong chromatographic performance for collagen characterization.

Chirality can mean the difference between medicine and catastrophe. In this article, we summarize the necessity of enantiomeric precision and explain how our Blossmate chiral columns offer flexible, MS-compatible solutions for analytical and preparative workflows, with chromatograms comparing our and competitor's column performances.

Retatrutide is a new synthetic peptide that facilitates weight loss through a dual-action mechanism. In this article, we utilize an Ultisil LP-C18 column for the determination of Retatrutide that achieves baseline separation with high resolution records.

Ultisil LP columns from Welch Materials are designed for low-pH chromatographic conditions, yet their advantages are more than simply resistance to strong acids. Read this article to learn how these columns outperform in a unique way.

This application note presents a robust SEC-HPLC method for high-resolution aggregation analysis of a Pembrolizumab biosimilar using Welch Xtimate™ SEC-300 columns. The method demonstrates excellent separation of monomer, HMW aggregates, and LMW fragments, supporting routine QC and biosimilar comparability studies.

Nicotine analysis is notoriously challenging due to its weak retention on conventional columns. This article shares a practical UHPLC-MS method using the Welch Ultisil HILIC Amphion II column, detailing the development approach, chromatographic conditions, and key advantages for reliable nicotine detection.

Mannitol is a substance with important applications in pharmaceutical and food. See how Welch Xtimate Sugar-Ca columns perform well in determination of mannitol related substances in accordance with Chinese Pharmacopoeia 2025 Edition.

This article explores how using Xtimate® Sugar-H and Sugar-Ca columns in series can significantly enhance resolution in sugar analysis. With real application cases in fermentation broth and pharmaceutical solutions, it demonstrates improved separation performance while maintaining safe pressure levels—offering a practical solution for challenging HPLC sugar separations.

In this article, we explore key considerations in gradient method development, and witness how optimizing gradient elution in HPLC is key to achieving efficient separation through a real-world case study.

In this article, we explore a simple yet effective trick in the elution program for isomer separation, with two examples to show how this trick works.