Knowledge Base
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A Premier Alkaloid: Nicotine and the Strategy for Developing Its Determination Method
Nicotine analysis is notoriously challenging due to its weak retention on conventional columns. This article shares a practical UHPLC-MS method using the Welch Ultisil HILIC Amphion II column, detailing the development approach, chromatographic conditions, and key advantages for reliable nicotine detection. -
Determination of Mannitol Related Substances with Xtimate Sugar-Ca
Mannitol is a substance with important applications in pharmaceutical and food. See how Welch Xtimate Sugar-Ca columns perform well in determination of mannitol related substances in accordance with Chinese Pharmacopoeia 2025 Edition. -
Sugar Columns in Series: Empowering Superior Separation Performance in Sugar Analysis
This article explores how using Xtimate® Sugar-H and Sugar-Ca columns in series can significantly enhance resolution in sugar analysis. With real application cases in fermentation broth and pharmaceutical solutions, it demonstrates improved separation performance while maintaining safe pressure levels—offering a practical solution for challenging HPLC sugar separations. -
Gradient Optimization in Liquid Chromatography
In this article, we explore key considerations in gradient method development, and witness how optimizing gradient elution in HPLC is key to achieving efficient separation through a real-world case study. -
A Simple Yet Effective Trick for Isomer Separation
In this article, we explore a simple yet effective trick in the elution program for isomer separation, with two examples to show how this trick works. -
Analytical Method for 18 Amino Acids
In this article, we share an application of an analytical method for 18 amino acids. Derivatization is employed in this method, and Welch Ultisil Amino Acid Column is used for analysis. -
Does Smaller Column Particle Size Always Lead to Better Resolution in Liquid Chromatography?
In this article, we explore how particle size affects resolution in liquid chromatography through two real-world applications, and analyze why these cases show different results from common belief. -
![[Reader Insight] Introduction to Common Derivatization Methods and Application Examples](//www.welch-us.com/cdn/shop/articles/derivatization-method-1080-1212_f33aec9a-97dd-4b57-8274-0c16be724f3f_345x345@2x.webp?v=1762501015)
[Reader Insight] Introduction to Common Derivatization Methods and Application Examples
This article explores key derivatization methods like methylation, amidation, and esterification, highlighting their principles, applications, and optimization strategies. -
Principles and Applications of Chaotropic Agents in the Analysis of Basic Compounds in RPLC
Learn about chaotropic agents in RPLC, their mechanisms, and benefits over ion-pair reagents, including improved retention, reproducibility, and column integrity. -
Unlocking the Potential of Derivatization Technology in HPLC Analysis
Discover how derivatization technology in HPLC boosts detection and separation performance, addressing analytical challenges with innovative techniques and real-world applications. -
Advanced Strategies for Retaining Polar Compounds in Chromatography: Case Studies and Practical Applications
Discover effective strategies and case studies for retaining polar compounds in chromatography, including column selection and practical applications. -
![[Reader Insight] A Guide to Selective Columns for Isomer Separation](//www.welch-us.com/cdn/shop/articles/selectivity-1080_a5bebdf8-ad89-4e8c-848d-f84a5656b179_345x345@2x.webp?v=1762503741)
[Reader Insight] A Guide to Selective Columns for Isomer Separation
Explore specialized chromatography columns with shape selectivity and spatial interactions for effective isomer separation, including phenyl/PFP, amide, and C8 columns, etc.
![[Reader Insight] Introduction to Common Derivatization Methods and Application Examples](http://www.welch-us.com/cdn/shop/articles/derivatization-method-1080-1212_f33aec9a-97dd-4b57-8274-0c16be724f3f_345x345@2x.webp?v=1762501015)
![[Reader Insight] A Guide to Selective Columns for Isomer Separation](http://www.welch-us.com/cdn/shop/articles/selectivity-1080_a5bebdf8-ad89-4e8c-848d-f84a5656b179_345x345@2x.webp?v=1762503741)