Ethinylestradiol on Ultisil XB-C4
Solution of test sample
1.1 Full scale
Gradient Program
| Time（min） | A% | B% |
| 0 | 100 | 0 |
| 35 | 100 | 0 |
| 65 | 0 | 100 |
| Column | Welch Ultisil XB-C4 4.6×250mm，5μm (P/N: 00216-31043) |
| Mobile Phase | A: Acetonitrile: water=30:70 B: Acetonitrile: water=75:25 |
| Detector | 220nm |
| Temperature | 30℃ |
| Flow Rate | 1.5ml/min |
| Injection Volume | 30ul |