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Adenine Isomer on Ultisil LP-C18

Redissolved solution(improve the speed to 1,5ml/min)

Column Welch Ultisil® LP-C18 4.6×150mm,5μm (P/N: 00208-31041)
Mobile Phase Dissolve 0.75g copper sulfate, 0.17g L-phenylalanine and 2.3g ammonium dihydrogen phosphate in 1000ml water Adjust pH to 4.0±0.05 with triethylamine
Detector UV 260 nm
Temperature 25°C
Flow Rate 0.5 ml/min
Injection Volume 10µl
Notes Use mobile phase(keep speed 0.2ml/min overnight)to balance column