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Polar compounds that refuse to stay on C18 columns can be frustrating. HILIC chromatography provides a versatile solution through partitioning and interaction mechanisms. In this post, we break down HILIC principles, column types, mobile phase strategies, and key method development tips to help you achieve reliable retention and separation.
![[Readers Insight] Hydrophilic Interaction Chromatography (HILIC): A Nemesis to Polar Compounds](//www.welch-us.com/cdn/shop/articles/1080_a134ed46-3aa6-4c67-878d-1c3786a088e9_535x.jpg?v=1767751595)
![[Readers Insight] Hydrophilic Interaction Chromatography (HILIC): A Nemesis to Polar Compounds](//www.welch-us.com/cdn/shop/articles/1080_a134ed46-3aa6-4c67-878d-1c3786a088e9_345x345@2x.jpg?v=1767751595)
![[Readers Insight] Hydrophilic Interaction Chromatography (HILIC): A Nemesis to Polar Compounds](http://www.welch-us.com/cdn/shop/articles/1080_a134ed46-3aa6-4c67-878d-1c3786a088e9_535x.jpg?v=1767751595)
![[Readers Insight] Hydrophilic Interaction Chromatography (HILIC): A Nemesis to Polar Compounds](http://www.welch-us.com/cdn/shop/articles/1080_a134ed46-3aa6-4c67-878d-1c3786a088e9_345x345@2x.jpg?v=1767751595)