Notice on Changes to pH Range Specifications for HPLC Columns
We would like to inform you of an important update to the pH range specifications for some of our HPLC column products. The pH range for these columns has been revised from 1.5–10 (for Topsil series, from 2.0–9.5) to 2.0–8.0.
Affected Products
A detailed table listing the affected columns and their updated specifications is provided below:
| Name | pH range | SSA | Aperture | Carbon Load % | USP | Max Temp | Max Puressure |
|---|---|---|---|---|---|---|---|
| Blossmate Reversed-Phase Column | |||||||
| Blossmate C4 | 2.0-8.0 | 15 | 450 | 0.5 | L26 | 60 | 40 |
| Blossmate Phenyl | 2.0-8.0 | 15 | 450 | 1 | L11 | 60 | 40 |
| Ultisil Reversed-Phase Column | |||||||
| Ultisil AQ-C18 |
2.0-8.0 |
320 | 120 | 12 | L1 | 60 (mobile phase pH ≤6.5) 40 (mobile phase pH >6.5) |
40 (≥5μm) 60 (＜5μm) |
| Ultisil XB-C18 |
2.0-8.0 |
320 | 120 | 17 | L1 | ||
| Ultisil XB-C18 |
2.0-8.0 |
90 | 300 | 8 | L1 | ||
| Ultisil Polar RP |
2.0-8.0 |
320 | 120 | 18 | L1/L60 | ||
| Ultisil PAH | 2.0-8.0 | 320 | 120 | 22 | L1 | ||
| Ultisil Alk C18 |
2.0-8.0 |
320 | 120 | 12 | L1 | ||
| Ultisil XS-C18 |
2.0-8.0 |
320 | 120 | 23 | L1 | ||
| Ultisil XB-C8 |
2.0-8.0 |
320 | 120 | 12 | L7 | ||
| Ultisil XB-C8 |
2.0-8.0 |
90 | 300 | 4 | L7 | ||
| Ultisil F-C8 | 2.0-8.0 | 320 | 120 | 12 | L7 | ||
| Ultisil Plus C8 | 2.0-8.0 | 160 | 130 | 7 | L7 | ||
| Ultisil XB-Phenyl | 2.0-8.0 | 320 | 120 | 12 | L11 | ||
| Ultisil Phenyl-Ether | 2.0-8.0 | 320 | 120 | 4 | L11 | ||
| Ultisil PFP | 2.0-8.0 | 320 | 120 | 12 | L11/L43 | ||
| Ultisil Plus Phenyl | 2.0-8.0 | 160 | 130 | 8 | L11 | ||
| Ultisil XB-C4 | 2.0-8.0 | 320 | 120 | 8 | L26 | ||
| Ultisil XB-C4 | 2.0-8.0 | 90 | 300 | 3 | L26 | ||
| Ultisil XB-C30 | 2.0-8.0 | 320 | 120 | 22 | L62 | ||
| Ultisil XB-C1 | 2.0-8.0 | 320 | 120 | 4 | L13 | ||
| Topsil Column | |||||||
| Topsil C18 | 2.0-8.0 | 260 | 150 | 12 | L1 | 60 (mobile phase pH ≤6.5) 40 (mobile phase pH >6.5) |
40 |
| Topsil C8 | 2.0-8.0 | 260 | 150 | 10 | L2 | ||
| Topsil Phenyl-Hexyl | 2.0-8.0 | 260 | 150 | 12 | L11 | ||
We are also in the process of reviewing and refining the specifications for additional columns. Further updates will be announced as soon as they are finalized.
Reason for the Change
The previous pH range was determined based on column stability at room temperature. However, chromatographic applications often operate at elevated temperatures, which can affect column durability. This adjustment ensures more accurate specifications for practical usage scenarios.
Technical Background
Silica-based stationary phases exhibit slight solubility in aqueous mobile phases when pH exceeds 8. To maximize the lifespan of silica-based columns, one should:
- Use buffers with concentrations not exceeding 20 mM.
- Maintain operating temperatures below 40°C.
Operating under extreme pH conditions or temperatures can significantly reduce column lifespan.
Thank you for your understanding and continued trust in our products. Should you have any questions, please feel free to contact our team.