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monoclonal, Agarose, Protein A Affinity Chromatography Resin

Monoclonal antibodies have uniform chemical properties and strong specificity, making them a widely used biopharmaceutical. Protein A affinity chromatography resin packing materials, due to its specific binding properties and powerful monoclonal antibody purification capabilities, has become an indispensable material in the production process of monoclonal antibody drugs.



Protein A Tanrose 4FF affinity chromatography medium


Welch Materials has utilized genetic recombination technology to redesign and develop a resilient alkaline-resistant, highly active protein A, and independently synthesized a high hydrophilicity, large pore size, high pressure-resistant, high capacity Protein A affinity chromatography product: Protein A Tanrose 4FF.



Protein A Tanrose 4FF affinity chromatography medium


The Protein A Tanrose 4FF chromatography medium introduced by Welch Materials utilizes high-flow agarose as a framework, with rigidity and mass transfer properties superior to traditional 4FF agarose frameworks.


Product Characteristics


01 Technical Parameters


Parameters Protein A Tanrose 4FF
Matrix Agarose
Ligand Alkaline-resistant recombinant Protein A
Ligand Binding Mode Epoxy Bonding
Particle Size ≈90μm
Dynamic Binding Capacity (5 min residence time) 40mg/mL hIgG
Maximum Pressure

0.5MPa
pH Stability

3-9 (long-term), 2-11 (short-term)
Maximum Flow Rate

300cm/h
Storage Conditions

2~8℃, 20% ethanol
CIP

0.1-0.5M NaOH

 02 High Dynamic Binding Capacity


Utilizing human immunoglobulin

Plasma-derived, purity 95%, concentration 1mg/mL.

Conducting binding capacity analysis

Column Volume: 1mL;

Retention Time: 5min;

Buffer A: 10mM PB + 0.15M NaCl, pH 7.2;

Buffer B: 0.1M Glycine, pH 3.0;

Flow Rate: 0.2mL/min;

Binding Capacity: Calculated based on breakthrough of 10%.

The dynamic binding curve of hIgG on alkaline-resistant Protein A medium with agarose matrix.


03 High Alkaline Resistance


Under conditions of 25℃, the "Protein A Tanrose 4FF" packing material was immersed in a 0.5M NaOH solution for 25 hours, and the change in dynamic binding capacity after alkaline immersion was tested. The results showed that the dynamic binding capacity of the packing material decreased by less than 10% after soaking in 0.5M sodium hydroxide for 25 hours.

The dynamic binding capacity change graph of the packing material after soaking in 0.5M sodium hydroxide for 25 hours is not provided.


04 The flow rate-pressure relationship.

The flow rate-pressure relationship graph of Protein A Tanrose 4FF affinity medium.

Packing Materials

Prepacked columns

Note:

Samples for all products in the order form are available for trial, and other specifications can be customized according to customer requirements.


We will soon launch a more powerful new generation of Protein A affinity chromatography products. Stay tuned!