Determination of mannitol content with Ultisil HILIC-Amide

Test Report

1. Experimental Process

1.1 Chromatographic Condition

1.2 Solution Preparation

1.2.1 Preparation of mobile phase:

Acetonitrile: Use an appropriate amount of chromatography-grade acetonitrile and degas with ultrasonication.

Water: Use an appropriate amount of ultrapure water and degas with ultrasonication.

1.2.2 Preparation of mannitol standard solution:

Weigh an appropriate amount of mannitol raw material provided by the client, dissolve in water to prepare a solution with a concentration of approximately 0.3 mg/mL, and mix well.

1.2.3 Preparation of sample solutions:

Weigh an appropriate amount of the sample provided by the customer, dissolve in water to prepare a solution with a concentration of approximately 5 mg/mL, mix well, filter, and use the filtrate.

2. Chromatogram and Data: 

2.1 Chromatogram of blank solvent:

2.2 Chromatogram of standard solution:

2.3 Chromatogram of sample solution 1 (for method development):

2.4 Chromatogram of sample solution 2 (for testing):

Using the Welch Ultisil® HILIC-Amide (4.6×250mm, 5μm) under these chromatographic conditions, the mannitol peak exhibits good shape and retention without interference from other substances, meeting the client’s detection requirements.