Doxycycline HCl on Xtimate PS DVB(EP7.0)
Sample
| Column | Xtimate® Phenyl-Hexyl, 7.8×250 mm, 8 μm |
| Mobile Phase | 50 g tert-butanol, 100mL water, 200 ml pH8.0 buffer, 10g/L tetrabutyl ammonium bisulfate solution(adjust pH to 8.0 with dilute sodium hydroxide solution) 25mL, 40g/L edta solution (adjust the pH of sodium solution to 8.0 with dilute hydroxide oxidation) 5mL, add water to 500mL |
| Detection | 254nm |
| Temperature | 75℃ |
| Flow Rate | 2.0 mL/min |
| Injection Volume | 20 µL |
| Notes | This method requires high control of column temperature |