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Clotrimazole on Ultisil XB-C18, Boltimate C18

Column: Ultisil XB-C18, 3.0×100mm, 1.8um

1.1. Reference Solution

(Flow Rate: 0.425ml/min; Injection Volume: 2.8ul; Initial Pressure: 427bar)

No. Component Rt (min) Half Width (min) Type Resolution (EP) Asymmetry (EP) Plates (EP)
1 Clotrimazole 11.208 0.188 M n.a. 1.09 19588
Mobile Phase Methanol : Buffer = 70 : 30, adjust pH to 5.7 with 10% phosphoric acid
Detection 215 nm
Temperature 30℃
Notes Column temperature shall be 30℃; Adjust the mobile phase pH after it’s well mixed.