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Clarithromycin on Ultisil Plus C18

Mixed standard solution

Gradient Program

Time(min) A% B%
0 70 30
32 40 60
34 40 60
36 75 25
45 75 25
Column Welch Ultisil Plus C18 4.6×250mm,5μm (P/N: 00260-31043)
Mobile Phase Mobile phase A: 4.76g/L potassium dihydrogen phosphate (pH4.4) Mobile phase B: Acetonitrile
Detector 205nm
Temperature 40℃
Flow Rate 1.1 ml/min
Injection Volume 10µl