Cefotiam Hydrochloride-related Substances on Xtimate C18
Content Determination
Column | Welch Xtimate C18 4.0x125mm,5μm (P/N: 00101-21031) |
Mobile Phase | Phosphate buffer (take about 800ml 0.05mol/l disodium hydrogen phosphate solution, adjust pH to 7.7 with 0.05mol/l potassium dihydrogen phosphate solution)-acetonitrile= 88:12 |
Detector | 254nm |
Temperature | 25℃ |
Flow Rate | 1ml/min |
Injection Volume | 20ul |