Cefotiam Hydrochloride on Xtimate C18

Column Welch Xtimate C18 4.0x125mm,5μm (P/N: 00101-21031)
Mobile Phase Acetonitrile-phosphate buffer solution (0.05 mol/L disodium hydrogen phosphate solution, pH adjusted to 7.7 with 0.05 mol/L potassium dihydrogen phosphate solution) = 12:88;
Detector 254nm
Temperature 25℃
Flow Rate 0.8ml/min
Injection Volume 10ul