Berberine Hydrochloride on Ultisil XB-C18
Mixed Reference
| Column | Ultisil XB-C18, 5μm, 4.6×250mm (P/N 00201-31043) |
| Mobile Phase | 0.01mol/L NH4H2PO4(adjust pH to 2.8 with phosphoric acid) : acetonitrile = 75:25 |
| Detection | 345nm |
| Temperature | 20℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | Column temperature has great influence on separation. |