Azithromycin on Ultisil Polar RP

Content determination of test sample

Column Welch Ultisil Polar RP 4.6×250mm,5μm (P/N: 00215-31043)
Mobile Phase 0.05 mol / L potassium dihydrogen phosphate solution(adjust pH to 8.2 with 20% phosphoric acid solution) :Acetonitrile = 45:55
Detector 210nm
Temperature 30℃
Flow Rate 1 ml/min
Injection Volume 50ul