Amikacin Sulfate on Ultisil Plus C18
Content ( Isocratic Elution)
Gradient Program （Related Substance）
| Time（min） | A% | B% |
| 0 | 62 | 38 |
| 30 | 62 | 38 |
| 60 | 0 | 100 |
| 70 | 0 | 100 |
| 71 | 62 | 38 |
| 80 | 62 | 38 |
| Column | Welch Ultisil Plus C18 4.6×250mm，5μm (P/N: 00260-31043) |
| Mobile Phase | A：0.2mol/L phosphate buffer（PH3.0） B: 0.2 mol/L potassium dihydrogen phosphate solution, adjust pH to 3.0 with 0.2 mol/L phosphoric acid |
| Isocratic Elution （Content Determination） | A:B=58：42 |
| Detector | 200nm |
| Temperature | 40℃ |
| Flow Rate | 1.3 ml/min |
| Injection Volume | 10µl |
| Notes | Peak of related substance appears at 26 min；Peak of content appears at 25 min. |