All-Trans-Astaxanthin on Ultisil AQ-C18
Assaying Reference Substance
| Column | Ultisil® XB-C30 4.6×250 mm, 5 μm P/N: 00223-31043 |
| Detection | 474 nm |
| Temperature | 30 ℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 µl |
| Notes | Sensitive to injection volume and dissolved solvent |
Mobile Phase
A: Methanol-1% Phosphoric acid solution=94:4 B: Methanol- Tert-butyl methyl ether- 1% Phosphoric acid solution=16:80:4
| Time | A% | B% |
| 0 | 67 | 33 |
| 15 | 52 | 48 |
| 23 | 0 | 100 |
| 27 | 67 | 33 |
| 30 | 67 | 33 |