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3 phosphoric acid sodium on Ultisil AQ-C18

Blank Solution

Column Ultisil® AQ-C18, 5μm, 4.6×250mm P/N: 00207-31043
Mobile Phase 0.2mol/L phosphate buffer (35.8g disodium hydrogen phosphate, 13.6g monopotassium phosphate, add 900mL water, adjust pH to 7.0 with 1mol/L NaOH, 1.61g tetrabutylammonium bromide. Add water to 1000mL)
Detection 271 nm
Temperature 35 ℃
Flow Rate 1.0 ml/min
Injection Volume 10 µl
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