P/N: H00201-31052

Ultisil XB-C18, 5μm, 7.8×300mm

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Ultisil XB-C18 is one of the most versatile C18 analytical columns on the market. It features advanced bonding and double-endcapping technology, delivering nearly perfect peak shapes. This column is ideal for analyzing complex matrix samples and provides excellent peak shapes for acidic and basic compounds. It is particularly effective for the separation of traditional Chinese medicine compounds.

Pairs well with

Ultisil Series HPLC Columns are based on ultra-pure ( purity > 99.999% ) spherical and totally porous silica, unique bonding chemistry and proprietary surface modification techniques, producing excellent peak shape, column efficiency and exceptional lot-to-lot reproducibility. Ultisil column is the best choice for method development, owing to complete bonding chemistries and stable performance.

Features

  • Ultra-pure spherical porous silica, purity > 99.999%
  • High efficiency: theoretical plate > 80000/m
  • Excellent peak symmetry: tailing factor=0.95~1.05
  • Exceptional lot-to-lot reproducibility

SEM Pictures of Ultisil Particles


Trace Amount Metal Contents Test

A useful chromatographic test of trace amount of metal contents in the column is to compare the peak symmetry of one pair of positional isomers, 4,4’-dipyridyl and 2,2’-dipyridyl, and a neutral chelating reagent, 1,2-dihydroxylnaphthalene. 4,4’-dipyridyl, which cannot form chelating complex with metal, is used as a reference. 2,2’-dipyridyl and 1,2-dihydroxylnaphthalene, which are chelating reagents, are sensitive to trace amount metal in silica. When a C18 column based on type A silica or other so-called type B silica with higher metal content is used, the peaks of 2,2’-dipyridyl and 1,2-dihydroxylnaphthalene would tail or even totally disappear.
Ultisil XB-C18 provides good peak shapes in the separation of these three compounds under pH 7.6, which indicates Ultisil silica contains hardly any metals.

Separation of Basic Compounds Antidepressant at pH 7.0

Comparison of Peak Shape between Batch to Batch

Capacity Factor(K’) of Batch to Batch Reproducibility

Brand: Ultisil

Phase: XB-C18

Particle Size: 5μm

Pore Size: 120Å

USP Classification: L1

Separation Mode: Reversed Mode

pH Range: 2.0-8.0

Surface Area: 320m2/g

Carbon Load: 17%

Max.Pressure: 40Mpa

Endcapped: Yes

Max.Temp.: 60°C(pH≤6.5) and 40°C(pH>6.5)

Particle Shape: Spherical

Similar Columns:

  • Agilent ZORBAX Eclipse XDB-C18
  • Agilent ZORBAX TYPE A Silica C18
  • Agilent Pursuit XRs C18
  • Agilent Pursuit XRs Ultra C18
  • Agilent HC (2)/TC (2) HC-C18 (2)
  • Phenomenex Columbus C18
  • Phenomenex Luna C18
  • Phenomenex Luna Omega C18
  • Phenomenex Prodigy ODS-2
  • Sepax Sepax GP-C18
  • Sepax Sepax Bio-C18-200
  • Sepax Sepax Sapphire C18
  • Supelco Discovery C18
  • Supelco Discovery HS C18
  • Thermo BETASIL C18
  • Thermo Fluophase RP 100A perfluorohexyl
  • Thermo Hypersil GOLD C18
  • Thermo Hypersil ODS (C18)
  • Thermo Hypersil ODS-2 (C18)
  • Thermo Acclaim 120 C18
  • Thermo Acclaim AMG C18
  • Thermo HyPURITY C18
  • Thermo HyPURITY Aquastar
  • Thermo Syncronis C18
  • Waters XBridge BEH 130-C18 (PST)
  • Waters XSelect HSS-C18
  • Waters Symmetry C18
  • Waters Sunfire C18
  • Waters Spherisorb ODS2 fully end capped
  • Waters Delta-Pak C18 (100A)
  • Waters BioSuite C18 PA-A

(Note: The above cross reference indicates alternative column(s) that will likely give a similar selectivity. There is no guarantee of 100% substitution and thus is for your reference only.)

Product Description

Ultisil Series HPLC Columns are based on ultra-pure ( purity > 99.999% ) spherical and totally porous silica, unique bonding chemistry and proprietary surface modification techniques, producing excellent peak shape, column efficiency and exceptional lot-to-lot reproducibility. Ultisil column is the best choice for method development, owing to complete bonding chemistries and stable performance.

Features

  • Ultra-pure spherical porous silica, purity > 99.999%
  • High efficiency: theoretical plate > 80000/m
  • Excellent peak symmetry: tailing factor=0.95~1.05
  • Exceptional lot-to-lot reproducibility

SEM Pictures of Ultisil Particles


Trace Amount Metal Contents Test

A useful chromatographic test of trace amount of metal contents in the column is to compare the peak symmetry of one pair of positional isomers, 4,4’-dipyridyl and 2,2’-dipyridyl, and a neutral chelating reagent, 1,2-dihydroxylnaphthalene. 4,4’-dipyridyl, which cannot form chelating complex with metal, is used as a reference. 2,2’-dipyridyl and 1,2-dihydroxylnaphthalene, which are chelating reagents, are sensitive to trace amount metal in silica. When a C18 column based on type A silica or other so-called type B silica with higher metal content is used, the peaks of 2,2’-dipyridyl and 1,2-dihydroxylnaphthalene would tail or even totally disappear.
Ultisil XB-C18 provides good peak shapes in the separation of these three compounds under pH 7.6, which indicates Ultisil silica contains hardly any metals.

Separation of Basic Compounds Antidepressant at pH 7.0

Comparison of Peak Shape between Batch to Batch

Capacity Factor(K’) of Batch to Batch Reproducibility

Specifications

Brand: Ultisil

Phase: XB-C18

Particle Size: 5μm

Pore Size: 120Å

USP Classification: L1

Separation Mode: Reversed Mode

pH Range: 2.0-8.0

Surface Area: 320m2/g

Carbon Load: 17%

Max.Pressure: 40Mpa

Endcapped: Yes

Max.Temp.: 60°C(pH≤6.5) and 40°C(pH>6.5)

Particle Shape: Spherical

Similar Columns:

  • Agilent ZORBAX Eclipse XDB-C18
  • Agilent ZORBAX TYPE A Silica C18
  • Agilent Pursuit XRs C18
  • Agilent Pursuit XRs Ultra C18
  • Agilent HC (2)/TC (2) HC-C18 (2)
  • Phenomenex Columbus C18
  • Phenomenex Luna C18
  • Phenomenex Luna Omega C18
  • Phenomenex Prodigy ODS-2
  • Sepax Sepax GP-C18
  • Sepax Sepax Bio-C18-200
  • Sepax Sepax Sapphire C18
  • Supelco Discovery C18
  • Supelco Discovery HS C18
  • Thermo BETASIL C18
  • Thermo Fluophase RP 100A perfluorohexyl
  • Thermo Hypersil GOLD C18
  • Thermo Hypersil ODS (C18)
  • Thermo Hypersil ODS-2 (C18)
  • Thermo Acclaim 120 C18
  • Thermo Acclaim AMG C18
  • Thermo HyPURITY C18
  • Thermo HyPURITY Aquastar
  • Thermo Syncronis C18
  • Waters XBridge BEH 130-C18 (PST)
  • Waters XSelect HSS-C18
  • Waters Symmetry C18
  • Waters Sunfire C18
  • Waters Spherisorb ODS2 fully end capped
  • Waters Delta-Pak C18 (100A)
  • Waters BioSuite C18 PA-A

(Note: The above cross reference indicates alternative column(s) that will likely give a similar selectivity. There is no guarantee of 100% substitution and thus is for your reference only.)

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