Effective Elimination of Ghost Peaks in HPLC Analysis
During HPLC separation, especially in gradient elution or when the system has been used for a long period, sporadic and unpredictable peaks (commonly referred to as “ghost peaks”) are likely to appear. The causes of ghost peaks vary, and the primary ones are impurities in the mobile phase and contamination within the instrument system.
At Welch Materials, we've launched the Ghost-Buster column. It effectively absorbs and removes impurities from the system, thereby preventing interference from impurity on the target peaks.
Product Features
Ghost-Buster Columns not only remove impurities from the mobile phase but also effectively capture contaminants within the plumbing and mixer, eliminating interferences from ghost peaks in analyses, enhancing workflow efficiency, and extending the lifespans of columns and instruments to some extent.
Through further upgrades and refinements, Ghost-Buster Columns II not only achieve superior adsorption of mobile-phase impurities to eliminate ghost peaks, but also address the baseline drift caused by an excessively high initial aqueous proportion in gradient runs, yielding a markedly more stable baseline.
Ghost-Buster UP Columns, rated to withstand pressures up to 90 MPa, offer seamless compatibility with UHPLC systems.
Application Case
| Column | Ultisil AQ-C18, 4.6×100 mm, 3 µm |
| Flow Rate | 1.0 mL/min |
| Volume | 5 µL |
| Wavelength | 210 nm |
| Temperature | 25℃ |
| Mobile Phase A | 0.05% Phosphoric acid |
| Mobile Phase B | Acetonitrile |
Installation
Install Ghost-Buster Columns between the gradient mixer and the autosampler, ensuring they are placed before the autosampler. If installed after the autosampler, the column may strongly adsorb the sample, leading to interference.
Precautions
- For a new column, flush with 80% methanol–in-water at 1.0 mL/min for 15 minutes before installation.
- Not all impurities present in the mobile phase can be adsorbed by the Ghost-Buster Column.
- When using ion-pairing reagents in the mobile phase, these reagents may be retained by the Ghost-Buster Column, potentially affecting analyte retention times or peak shapes. Under such conditions, evaluate chromatographic performance to determine whether to employ the Ghost-Buster Column.
- If adsorption efficiency declines, replace the column promptly; it is not intended for unlimited reuse.
Frequently Asked Questions
Q: For different samples and gradient conditions, should the Ghost-Buster column be removed or changed?
A: Not necessary. But it needs to be removed only for special circumstances like changing of peak position or ion-pair solvents mobile phase.
Q: When gradient elution changed to isocratic, should the Ghost-Buster column be removed?
A: No need to take the Ghost-Buster column if it did not affect the separation, as the elution of mobile phase stays same under isocratic condition. But impurities in mobile phase shall be taken into consideration.
Q: In gradient system, Ghost-Buster column increases the mixed dwell volume. Will this affect the separation?
A: The packing volume of a 4.6x50mm column is ~400ul and the column is installed before the injector, which would cause little influence on the analysis. lf it does, connect Ghost-Buster column to the water phase path before the mixer or switching valve.
Q: Any requirements for the connecting of Ghost-Buster column?
A: No special requirements for the connection, Common PEEK tube and connectors for HPLC system is recommended.
ID: 2.1mm
Length: 30mm
Max.Pressure: 90Mpa
Effective Elimination of Ghost Peaks in HPLC Analysis
During HPLC separation, especially in gradient elution or when the system has been used for a long period, sporadic and unpredictable peaks (commonly referred to as “ghost peaks”) are likely to appear. The causes of ghost peaks vary, and the primary ones are impurities in the mobile phase and contamination within the instrument system.
At Welch Materials, we've launched the Ghost-Buster column. It effectively absorbs and removes impurities from the system, thereby preventing interference from impurity on the target peaks.
Product Features
Ghost-Buster Columns not only remove impurities from the mobile phase but also effectively capture contaminants within the plumbing and mixer, eliminating interferences from ghost peaks in analyses, enhancing workflow efficiency, and extending the lifespans of columns and instruments to some extent.
Through further upgrades and refinements, Ghost-Buster Columns II not only achieve superior adsorption of mobile-phase impurities to eliminate ghost peaks, but also address the baseline drift caused by an excessively high initial aqueous proportion in gradient runs, yielding a markedly more stable baseline.
Ghost-Buster UP Columns, rated to withstand pressures up to 90 MPa, offer seamless compatibility with UHPLC systems.
Application Case
| Column | Ultisil AQ-C18, 4.6×100 mm, 3 µm |
| Flow Rate | 1.0 mL/min |
| Volume | 5 µL |
| Wavelength | 210 nm |
| Temperature | 25℃ |
| Mobile Phase A | 0.05% Phosphoric acid |
| Mobile Phase B | Acetonitrile |
Installation
Install Ghost-Buster Columns between the gradient mixer and the autosampler, ensuring they are placed before the autosampler. If installed after the autosampler, the column may strongly adsorb the sample, leading to interference.
Precautions
- For a new column, flush with 80% methanol–in-water at 1.0 mL/min for 15 minutes before installation.
- Not all impurities present in the mobile phase can be adsorbed by the Ghost-Buster Column.
- When using ion-pairing reagents in the mobile phase, these reagents may be retained by the Ghost-Buster Column, potentially affecting analyte retention times or peak shapes. Under such conditions, evaluate chromatographic performance to determine whether to employ the Ghost-Buster Column.
- If adsorption efficiency declines, replace the column promptly; it is not intended for unlimited reuse.
Frequently Asked Questions
Q: For different samples and gradient conditions, should the Ghost-Buster column be removed or changed?
A: Not necessary. But it needs to be removed only for special circumstances like changing of peak position or ion-pair solvents mobile phase.
Q: When gradient elution changed to isocratic, should the Ghost-Buster column be removed?
A: No need to take the Ghost-Buster column if it did not affect the separation, as the elution of mobile phase stays same under isocratic condition. But impurities in mobile phase shall be taken into consideration.
Q: In gradient system, Ghost-Buster column increases the mixed dwell volume. Will this affect the separation?
A: The packing volume of a 4.6x50mm column is ~400ul and the column is installed before the injector, which would cause little influence on the analysis. lf it does, connect Ghost-Buster column to the water phase path before the mixer or switching valve.
Q: Any requirements for the connecting of Ghost-Buster column?
A: No special requirements for the connection, Common PEEK tube and connectors for HPLC system is recommended.
ID: 2.1mm
Length: 30mm
Max.Pressure: 90Mpa